Buitrago F, Canales J, Sillero A
Bioquímica y Biología Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
Anal Biochem. 1988 Jun;171(2):389-92. doi: 10.1016/0003-2697(88)90502-7.
Commercial samples of GTP and guanosine 5'-tetraphosphate were analyzed, with or without previous treatment with alkaline phosphatase, by high-pressure liquid chromatography on a Hypersil ODS column. They showed the presence of diguanosine 5',5"'-Pl,Pn-tri, tetra-, and pentaphosphates in varying amounts depending on the sample, but usually in proportions of around 0.3%.
对鸟苷三磷酸(GTP)和5'-四磷酸鸟苷的商业样品进行了分析,无论是否预先用碱性磷酸酶处理,均在Hypersil ODS柱上通过高压液相色谱法进行分析。结果显示,根据样品不同,存在不同含量的5',5"'-P1,Pn-三磷酸、四磷酸和五磷酸二鸟苷,但通常比例约为0.3%。