Manoil C, Kaiser D
J Bacteriol. 1980 Jan;141(1):297-304. doi: 10.1128/jb.141.1.297-304.1980.
Cultures of Myxococcus xanthus develop multicellular fruiting bodies when starved for carbon and nitrogen sources on an agar surface. Under these conditions of severe starvation, cultures rapidly accumulated a compound identified as guanosine tetraphosphate by chromatographic migration of the compound and of its major acid and alkali breakdown products. The accumulation of guanosine tetraphosphate was reduced in the presence of tetracycline, indicating that it may be synthesized by mechanisms similar to those of Escherichia coli. The guanosine tetraphosphate level was also reduced in starved cultures of a mutant unable to fruit normally, although it has been determined whether the defect in guanosine tetraphosphate accumulation is responsible for the inability to fruit. Induction of spores by glycerol addition led to transient increases in both guanosine tetraphosphate and guanosine pentaphosphate at a stage following most cell shortening, but before spores had acquired full refractility.
当黄色粘球菌在琼脂表面缺乏碳源和氮源而饥饿时，其培养物会形成多细胞子实体。在这种严重饥饿的条件下，培养物迅速积累了一种化合物，通过该化合物及其主要酸碱分解产物的色谱迁移鉴定为四磷酸鸟苷。在四环素存在的情况下，四磷酸鸟苷的积累减少，这表明它可能通过与大肠杆菌类似的机制合成。在一个不能正常形成子实体的突变体的饥饿培养物中，四磷酸鸟苷水平也降低了，尽管尚未确定四磷酸鸟苷积累缺陷是否是导致不能形成子实体的原因。添加甘油诱导孢子形成，在大多数细胞缩短之后、孢子获得完全折光性之前的一个阶段，四磷酸鸟苷和五磷酸鸟苷都会短暂增加。
