使用紫外可见光谱法测定埃塞俄比亚哈布鲁县周边生咖啡豆和烘焙咖啡豆中咖啡因和过氧化氢的抗氧化活性

Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy.

作者信息

Legas Muhammed Beshir, Hussen Seid Mohammed, Habte Adere Tarekegne

机构信息

Department of Chemistry, Woldia University, Woldia, Ethiopia.

出版信息

Clin Pharmacol. 2021 May 24;13:101-113. doi: 10.2147/CPAA.S311032. eCollection 2021.

DOI:10.2147/CPAA.S311032

PMID:34079391

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8163633/

Abstract

BACKGROUND

Coffee is a well-known beverage that is widely used around the world. Despite the wide use of coffee in Ethiopia, there is a lack of extensive studies addressing the issues related to the caffeine content and hydrogen peroxide antioxidant activity of varieties of coffee types, particularly in Habru woreda, Ethiopia.

OBJECTIVE

This study aimed to determine the caffeine content and hydrogen peroxide antioxidant activity of raw and roasted coffee beans collected directly from Habru woreda, North Wollo zone of Ethiopia.

METHODS

The study was conducted in Bohoro, Girana, and Wurgisa kebeles of Habru woreda, Ethiopia, by collecting 500 g of green beans of Arabica coffee without considering their variety. Then, the collected beans were divided into raw and roasted coffee to perform aqueous and dichloromethane extraction of their caffeine content and hydrogen peroxide antioxidant activity using UV-Vis spectrophotometry.

RESULTS

The amounts of caffeine in aqueous and dichloromethane extraction were in the range of 124.01-191.27 ppm and 145.15-200.09 ppm in raw and roasted coffees, respectively. Using the IC value, the hydrogen peroxide scavenging activity of the aqueous phase coffee bean extracts in Bohoro raw, Bohoro roasted, Wurgisa raw, Wurgisa roasted, Girana raw, and Girana roasted coffee were 32.17 ppm, 11.69 ppm, 26.14 ppm, 3.12 ppm, 24.83 ppm, and 11.06 ppm, respectively, while that of ascorbic acid was 6.91 ppm.

CONCLUSION

The study showed that the highest amount of caffeine in both aqueous and dichloromethane solvent extraction was found in Bohoro's raw and roasted coffee beans. Also, the amounts of caffeine in all coffee bean samples were safe and the antioxidant activity was excellent. In most of the samples, significant variations in the concentration of caffeine in raw and roasted coffee bean samples were observed in the two extraction solvents.

摘要

背景

咖啡是一种广为人知的饮品，在全球被广泛饮用。尽管咖啡在埃塞俄比亚广泛使用，但缺乏针对各类咖啡品种的咖啡因含量和过氧化氢抗氧化活性相关问题的广泛研究，尤其是在埃塞俄比亚北沃洛州的哈布鲁县。

目的

本研究旨在测定直接从埃塞俄比亚北沃洛州哈布鲁县采集的生咖啡豆和烘焙咖啡豆的咖啡因含量及过氧化氢抗氧化活性。

方法

该研究在埃塞俄比亚哈布鲁县的博霍罗、吉拉纳和乌尔吉萨乡进行，采集了500克阿拉比卡咖啡豆，未考虑其品种。然后，将采集的咖啡豆分为生咖啡和烘焙咖啡，使用紫外可见分光光度法对其咖啡因含量和过氧化氢抗氧化活性进行水相和二氯甲烷萃取。

结果

生咖啡和烘焙咖啡的水相和二氯甲烷萃取物中的咖啡因含量分别在124.01 - 191.27 ppm和145.15 - 200.09 ppm范围内。利用IC值，博霍罗生咖啡、博霍罗烘焙咖啡、乌尔吉萨生咖啡、乌尔吉萨烘焙咖啡、吉拉纳生咖啡和吉拉纳烘焙咖啡的水相咖啡豆提取物的过氧化氢清除活性分别为32.17 ppm、11.69 ppm、26.14 ppm、3.12 ppm、24.83 ppm和11.06 ppm，而抗坏血酸的为6.91 ppm。