Programa de Pós-graduação em Ciência e Engenharia de Materiais (PPGCEM), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brasil.
Laboratório de Síntese de Nanomateriais e Nanoscopia (LSNN), Universidade Federal do Amazonas (UFAM), Manaus, Amazonas, Brasil.
Nanotechnology. 2021 Jun 3;32(34). doi: 10.1088/1361-6528/ac027e.
Increasing interest in nanoparticles of technological application has been improving their fabrication processes. The encapsulation of essential oils as bioactive compounds has proved to be an excellent alternative to the use of less environment friendly compounds. However, the difficulty of identifying their constitution and interaction with carrier agents have aroused scientific interest and a problem to overcome. Bilayer-based nanoparticles were developed using gelatin and poly--caprolactone (PCL) aiming the encapsulation ofessential oil. based on atomic force microscopy images and dynamic light scattering analysis, the size of the unloaded and loaded nanoparticles was found around (194 ± 40) and (296 ± 54) nm, respectively. The spatial patterns revealed that the surface of nanoparticles presented different surface roughness, similar shapes and height distribution asymmetry, lower dominant spatial frequencies, and different spatial complexity. Traditional infrared spectroscopy allowed the identification of the nanoparticle outermost layer formed by the gelatin carrier, but microscopy-based infrared spectroscopy revealed a band at 1742 cmrelated to the carbonyl stretching mode of PCL, as well as a band at 1557 cmdue to the amide II group from gelatin. The combination of microscopy and spectroscopy techniques proved to be an efficient alternative to quickly identify differences in chemical composition by evaluating different functional groups in bilayer PLC/gelatin nanoparticles of technological application.
对具有技术应用的纳米粒子的兴趣日益增加，这促使人们改进其制造工艺。将精油包封作为生物活性化合物已被证明是替代使用环境不友好化合物的绝佳选择。然而，识别其组成和与载体相互作用的困难引起了科学界的兴趣，也是需要克服的一个问题。本研究采用明胶和聚己内酯（PCL）制备了双层纳米粒子，旨在包封精油。基于原子力显微镜图像和动态光散射分析，未加载和加载纳米粒子的粒径分别约为（194±40）和（296±54）nm。空间模式表明，纳米粒子的表面呈现出不同的表面粗糙度、相似的形状和高度分布不对称性、较低的主导空间频率以及不同的空间复杂性。传统的红外光谱允许识别由明胶载体形成的纳米粒子最外层，但基于显微镜的红外光谱显示出与 PCL 羰基伸缩模式相关的 1742 cm处的带以及与明胶的酰胺 II 基团相关的 1557 cm处的带。显微镜和光谱技术的结合被证明是一种有效的替代方法，可以通过评估技术应用的双层 PLC/明胶纳米粒子中的不同官能团，快速识别化学成分的差异。
