基于单个量子点的纳米传感器的锆离子介导组装用于激酶分析。

Zirconium ion-mediated assembly of a single quantum dot-based nanosensor for kinase assay.

机构信息

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.

Department of Internal Medicine, Affiliated Cancer Hospital of Zhengzhou University, Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450000, China.

出版信息

Chem Commun (Camb). 2021 Jun 29;57(52):6376-6379. doi: 10.1039/d1cc02035h.

DOI:10.1039/d1cc02035h

PMID:34081069

Abstract

We demonstrate the zirconium ion-mediated assembly of a single quantum dot (QD)-based nanosensor for accurate detection of protein kinases (PKA) and polynucleotide kinases (PNK). This nanosensor is very sensitive with a detection limit of 8.82 × 10-4 U mL-1 for PKA and 1.40 × 10-5 U mL-1 for PNK. Moreover, it can be used to analyze the enzyme kinetic parameters and screen the inhibitors of PKA and PNK, with potential applications in drug discovery and clinical diagnosis.

摘要

我们展示了一种基于单量子点的纳米传感器，该传感器通过锆离子介导的组装，可以准确检测蛋白激酶（PKA）和多核苷酸激酶（PNK）。这种纳米传感器具有非常高的灵敏度，对 PKA 的检测限为 8.82×10-4 U mL-1，对 PNK 的检测限为 1.40×10-5 U mL-1。此外，它还可以用于分析酶的动力学参数，并筛选 PKA 和 PNK 的抑制剂，具有在药物发现和临床诊断中的潜在应用。