Harris S C, Miller M A, Wallace J E
Department of Pathology, Audie L. Murphy Memorial Veterans Administration Hospital, San Antonio, TX 78284.
Ann Clin Lab Sci. 1988 Jul-Aug;18(4):297-305.
A method is described for the determination of codeine and its metabolite, morphine, at low nanogram concentrations in plasma. Analysis is accomplished by high-performance liquid chromatography utilizing a cyanopropyl normal bonded phase (NBP) column in reversed-phase mode and two electrochemical detectors in series configuration. Two internal standards are utilized, ethyl morphine for codeine and nalorphine for morphine. Codeine and morphine concentration data are presented for several patients receiving codeine-containing medications. The lower limit of detectability was 2.00 +/- 0.39 ng per mL for codeine and 1.20 +/- 0.83 ng per mL for morphine. The patient sample mean within-run coefficients of variation for codeine and morphine (at 10 ng per mL) were less than 10 percent, n = 30. The between-run coefficient of variation for codeine was also less than 10 percent (over a range of two to 190 ng per mL, n = 61), and was approximately 15 percent for morphine (over a range of two to 40 ng per mL, n = 31).
本文描述了一种测定血浆中低纳克浓度可待因及其代谢物吗啡的方法。分析通过高效液相色谱法完成，采用氰丙基正相键合相（NBP）柱进行反相模式，并串联两个电化学检测器。使用了两种内标，乙基吗啡用于可待因，烯丙吗啡用于吗啡。给出了几名接受含可待因药物治疗患者的可待因和吗啡浓度数据。可待因的检测下限为每毫升2.00±0.39纳克，吗啡为每毫升1.20±0.83纳克。可待因和吗啡（浓度为每毫升10纳克时）的患者样本批内变异系数小于10%，n = 30。可待因的批间变异系数也小于10%（浓度范围为每毫升2至190纳克，n = 61），吗啡的批间变异系数约为15%（浓度范围为每毫升2至40纳克，n = 31）。
