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炭疽菌属 VOCs 特征可作为一种评价针叶樱桃浆质量控制的潜在工具。

VOCs profile of Colletotrichum spp. as a potential tool for quality control of açaí pulp.

机构信息

Instituto de Ciências Exatas, Universidade Federal do Sul e Sudeste do Pará, Marabá-PA 68505-080, Brazil.

Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS 90040-060, Brazil.

出版信息

Food Chem. 2021 Nov 15;362:130150. doi: 10.1016/j.foodchem.2021.130150. Epub 2021 May 18.

DOI:10.1016/j.foodchem.2021.130150
PMID:34082295
Abstract

Colletotrichum has been identified as responsible for the "dried fruit" disease in açaí (Euterpe oleracea). Besides concern for açaí pulp quality control, the characterization of Colletotrichum has been difficult, which has motivated the search for chemical markers in the Volatile Organic Compounds (VOCs) profile for use as a tool in the identification of açaí pulp contaminated by the fungus. Extracted VOCs by Headspace - Solid Phase Micro Extraction (HS-SPME) were identified through gas chromatography mass spectrometry (GC-MS). From GC-MS analyzes 26 VOCs were identified, with a predominance of the terpenoids. Chemometrically, menthol and menthone were assigned as potential markers of the genus. The analysis of VOCs in açaí pulps contaminated by Colletotrichum under different cultivation conditions enabled the detection of menthone. This result illustrated the selectivity of the culture medium and the potential of this tool for use in the quality control of açaí pulp.

摘要

炭疽菌已被确定是导致巴西莓(Euterpe oleracea)“干果”病的原因。除了关注巴西莓果肉的质量控制外,炭疽菌的特征描述一直很困难,这促使人们在挥发性有机化合物(VOCs)谱中寻找化学标记物,作为鉴定受真菌污染的巴西莓果肉的工具。通过顶空固相微萃取(HS-SPME)提取的 VOCs 通过气相色谱质谱联用仪(GC-MS)进行鉴定。通过 GC-MS 分析鉴定出 26 种 VOC,其中萜类化合物占优势。通过化学计量学分析,薄荷醇和薄荷酮被指定为该属的潜在标记物。对不同培养条件下受炭疽菌污染的巴西莓果肉中的 VOCs 进行分析,检测到了薄荷酮。这一结果说明了培养基的选择性和该工具在巴西莓果肉质量控制中的应用潜力。

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