Department of Gastroenterology, Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan 250101, China.
Department of Endocrinology and Metabolism, Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan 250101, China.
J Biomed Nanotechnol. 2021 May 1;17(5):889-900. doi: 10.1166/jbn.2021.3079.
In the current study, we hypothesized that the electrospun scaffold chitosan (CS)/polycaprolactone (PCL)/titanium dioxide (TiO₂) could be prepared by combining CS, PCL, and TiO₂ nanoparticles (TiO₂ NPs) using an electrospinning technique for wound dressing applications. The CS/PCL/TiO₂ electrospun scaffold was prepared and characterized by UV-Vis, SEM, TEM, FTIR, and XRD analyses. Based on the UV-Vis analysis, the incorporation of CS/PCL on the surface of TiO₂ NPs affected their optical properties. Further, CS/PCL and CS/PCL/TiO₂ were found to have uniform distribution in fiber diameter with no bead morphology, as confirmed by SEM. The XRD spectrum of the CS/PCL/TiO₂ revealed that the TiO₂ NPs were adequately mixed with the CS/PCL solution, exhibiting the planes of TiO₂ peaks (112), (105), (204), (116), and (301), which aligned well with the lattice structure. The antibacterial activity of CS/PCL/TiO₂ against and was evaluated using the zone of inhibition method. By testing the cytocompatibility of CS/PCL/TiO₂ this dressing was found to have a less toxic nature. In addition, wound healing studies showed that the dressing prepared with the CS/PCL/TiO₂ electrospun scaffold improved wound healing compared to that prepared with CS/PCL alone. The above results strongly support the use of CS/PCL/TiO₂ electrospun scaffold as an effective dressing for wound healing.
在当前的研究中，我们假设通过电纺技术将壳聚糖（CS）/聚己内酯（PCL）/二氧化钛（TiO₂）与 CS、PCL 和 TiO₂纳米粒子（TiO₂ NPs）结合在一起，可以制备 CS/PCL/TiO₂电纺支架，用于伤口敷料应用。通过 UV-Vis、SEM、TEM、FTIR 和 XRD 分析对 CS/PCL/TiO₂电纺支架进行了制备和表征。基于 UV-Vis 分析，CS/PCL 对 TiO₂ NPs 表面的结合影响了它们的光学性质。此外，CS/PCL 和 CS/PCL/TiO₂被发现具有均匀的纤维直径分布，没有珠状形态，这通过 SEM 得到了证实。CS/PCL/TiO₂的 XRD 谱表明，TiO₂ NPs 与 CS/PCL 溶液充分混合，表现出 TiO₂峰的平面（112）、（105）、（204）、（116）和（301），与晶格结构吻合良好。通过抑菌圈法评估 CS/PCL/TiO₂对 和 的抗菌活性。通过测试 CS/PCL/TiO₂ 的细胞相容性，发现这种敷料具有较低的毒性。此外，伤口愈合研究表明，与单独使用 CS/PCL 制备的敷料相比，用 CS/PCL/TiO₂电纺支架制备的敷料可改善伤口愈合。上述结果有力地支持了将 CS/PCL/TiO₂电纺支架用作有效的伤口愈合敷料的使用。
