College of Textiles and Clothing, Qingdao University, Qingdao 266071, Shandong, China.
College of Textiles and Clothing, Qingdao University, Qingdao 266071, Shandong, China.
Int J Biol Macromol. 2022 Apr 30;205:500-510. doi: 10.1016/j.ijbiomac.2022.02.118. Epub 2022 Feb 23.
The aim of this study was to optimize the chitosan/polycaprolactone (CS/PCL) electrospun nanofibrous membrane with random/aligned fiber structures to provide a controlled release of ciprofloxacin (Cip) and guide skin fibroblasts arrangement. A series of Cip-encapsulated CS/PCL electrospun membranes were prepared by coaxial-electrospinning. The existence of Cip in core-shell structured fibers was confirmed by using SEM, TEM and FTIR characterizations. The in vitro drug-release profiles suggested that the Cip presented a sustained release for 15 days. Simultaneously, cyto-compatibility of the membranes decreased with the increasing amount of Cip from 2.0% to 5.0%. In particular, aligned CS/PCL membrane loading with 2.0% Cip exhibited a good balanced ability between cell proliferation and antibacterial effect (>99% against Escherichia coli and Staphylococcus aureus), which significantly accelerated the wound healing process in vivo. These results suggested that the aligned CS/PCL membrane loading with 2.0% Cip exhibited great antibacterial property and biocompatibility, which possess promising applications potential for wound healing.
本研究旨在通过优化壳聚糖/聚己内酯（CS/PCL）随机/定向纤维结构的静电纺丝纳米纤维膜，实现环丙沙星（Cip）的控制释放并引导皮肤成纤维细胞排列。通过同轴静电纺丝制备了一系列载有 Cip 的 CS/PCL 静电纺丝膜。SEM、TEM 和 FTIR 表征证实了芯壳结构纤维中 Cip 的存在。体外药物释放曲线表明，Cip 持续释放 15 天。同时，随着 Cip 含量从 2.0%增加到 5.0%，膜的细胞相容性逐渐降低。特别是载有 2.0% Cip 的定向 CS/PCL 膜在细胞增殖和抗菌效果之间表现出良好的平衡能力（对大肠杆菌和金黄色葡萄球菌的抑菌率均大于 99%），这显著加速了体内的伤口愈合过程。这些结果表明，载有 2.0% Cip 的定向 CS/PCL 膜具有良好的抗菌性能和生物相容性，在伤口愈合方面具有广阔的应用前景。
