AntiCancer, Inc., San Diego, CA, USA.
Adv Drug Deliv Rev. 2013 Mar;65(3):383-90. doi: 10.1016/j.addr.2012.08.007. Epub 2012 Aug 17.
Exosomes play an important role in cell-to-cell communication to promote tumor metastasis. In order to image the fate of cancer-cell-derived exosomes in orthotopic nude mouse models of breast cancer, we used green fluorescent protein (GFP)-tagged CD63, which is a general marker of exosomes. Breast cancer cells transferred their own exosomes to other cancer cells and normal lung tissue cells in culture. In orthotopic nude-mouse models, breast cancer cells secreted exosomes into the tumor microenvironment. Tumor-derived exosomes were incorporated into tumor-associated cells as well as circulating in the blood of mice with breast cancer metastases. These results suggest that tumor-derived exosomes may contribute to forming a niche to promote tumor growth and metastasis. Our results demonstrate the usefulness of GFP imaging to investigate the role of exosomes in cancer metastasis.
外泌体在细胞间通讯中发挥着重要作用，促进肿瘤转移。为了在乳腺癌原位裸鼠模型中对癌细胞衍生的外泌体的命运进行成像，我们使用了绿色荧光蛋白 (GFP) 标记的 CD63，它是外泌体的通用标志物。乳腺癌细胞在培养中将自身的外泌体转移到其他癌细胞和正常肺组织细胞中。在原位裸鼠模型中，乳腺癌细胞将外泌体分泌到肿瘤微环境中。肿瘤衍生的外泌体被纳入肿瘤相关细胞，并在乳腺癌转移小鼠的血液中循环。这些结果表明，肿瘤衍生的外泌体可能有助于形成一个小生境，促进肿瘤生长和转移。我们的结果表明 GFP 成像在研究外泌体在癌症转移中的作用方面是有用的。
