Baussant T, Michaud A, Bouhnik J, Savoie F, Alhenc-Gelas F, Corvol P
INSERM U36-17, Paris, France.
Biochem Biophys Res Commun. 1988 Aug 15;154(3):1160-6. doi: 10.1016/0006-291x(88)90262-8.
T Kininogen and High Molecular Weight Kininogen were characterized in the cell culture medium of Fao cells, a highly differentiated cell line derived from the Reuber H35 rat hepatoma. Immunoreactive T Kininogen and High Molecular Weight Kininogen identified by direct and specific RIAs were indistinguishable from standard kininogens. Immunoreactive T Kininogen was further identified by HPLC analysis of T kinin released after trypsin hydrolysis of the cell culture medium. The basal release rate of T kininogen was ten-fold higher than that of High Molecular Weight Kininogen. T Kininogen was not stored within the cells contrary to High Molecular Weight Kininogen. The production of the two kininogens in the cell medium was stimulated by dexamethasone up to five times in a dose-dependent manner. The specific antiglucocorticoid compound RU 38486 did not alter the basal rate of kininogen release by Fao cells, but abolished the stimulation by dexamethasone, indicating that dexamethasone exerts a true glucocorticoid type effect.
T激肽原和高分子量激肽原在Fao细胞的细胞培养基中得到了表征，Fao细胞是一种源自鲁伯H35大鼠肝癌的高度分化细胞系。通过直接和特异性放射免疫分析法鉴定的免疫反应性T激肽原和高分子量激肽原与标准激肽原无法区分。通过对细胞培养基胰蛋白酶水解后释放的T激肽进行高效液相色谱分析，进一步鉴定了免疫反应性T激肽原。T激肽原的基础释放率比高分子量激肽原高十倍。与高分子量激肽原相反，T激肽原不储存在细胞内。地塞米松以剂量依赖性方式刺激细胞培养基中两种激肽原的产生，最高可达五倍。特异性抗糖皮质激素化合物RU 38486不会改变Fao细胞激肽原释放的基础速率，但会消除地塞米松的刺激作用，表明地塞米松发挥了真正的糖皮质激素类型效应。
