Jacob B, Thierry D, Françoise S, Alhenc-Gelas F, Francis G, Frédéric E
Biochem Biophys Res Commun. 1987 Apr 29;144(2):1090-7. doi: 10.1016/s0006-291x(87)80076-1.
Antibodies raised in rabbits against pure rat T-kininogen did not cross-react with Ile-Ser-Bradykinin, bradykinin, nor with kininogens from other mammalian species. They presented a 1 to 15% cross-reaction with pure rat HMW kininogen, depending on the quantity of HMW kininogen. A direct radioimmunoassay for rat T-kininogen in plasma was developed and it enabled 89 fmol of the protein to be detected. A good correspondence was obtained between the direct RIA and the T-kinin generating assay. By the direct assay, it was found that T-kininogen is increased about ten fold in rats subcutaneously injected with turpentine. These data were confirmed by HPLC analysis of the plasma kinins released by trypsin which demonstrated that only T-kinins are increased, bradykinin being unchanged. It was possible according to the results obtained by the direct RIA and HPLC analysis to estimate that in the normal rat, HMW and LMW kininogen represent about 35% and T-kininogen 65%. In the turpentine-treated rat, T-kininogen reached 95%. This RIA will allow the study of the regulation of T-kininogen in the rat and the synthesis of this protein in cells in culture.
用纯大鼠T-激肽原免疫家兔产生的抗体，与异亮氨酸-丝氨酸-缓激肽、缓激肽以及其他哺乳动物物种的激肽原均无交叉反应。它们与纯大鼠高分子量激肽原存在1%至15%的交叉反应，具体取决于高分子量激肽原的量。开发了一种用于检测血浆中大鼠T-激肽原的直接放射免疫分析方法，该方法能够检测到89飞摩尔的该种蛋白质。直接放射免疫分析与T-激肽生成分析之间具有良好的一致性。通过直接分析发现，皮下注射松节油的大鼠体内T-激肽原增加了约10倍。胰蛋白酶释放的血浆激肽的高效液相色谱分析证实了这些数据，该分析表明只有T-激肽增加，缓激肽无变化。根据直接放射免疫分析和高效液相色谱分析的结果，可以估计在正常大鼠中，高分子量和低分子量激肽原约占35%，T-激肽原占65%。在松节油处理的大鼠中，T-激肽原达到95%。这种放射免疫分析将有助于研究大鼠体内T-激肽原的调节以及该蛋白在培养细胞中的合成。
