Zhang Xianglun, Zhao Hongbo, Sheng Qingkai, Liu Xiaomu, You Wei, Lin Haichao, Liu Guifen
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Shandong Key Lab of Animal Disease Control and Breeding, Jinan, China.
Arch Anim Breed. 2021 Mar 19;64(1):103-108. doi: 10.5194/aab-64-103-2021. eCollection 2021.
MicroRNAs are short non-coding RNAs that regulate gene expression. Several microRNAs, useful for coronary artery disease assessment, have previously been identified. MicroRNA-33 is located within SREBP introns and controls cholesterol homeostasis. In order to find the possibility of microRNA-33 as a potential biomarker in high cholesterol disease, we developed a mouse model for coronary heart disease by feeding mice with a high-fat diet. The expression differences of microRNA-33, SREBP and ABCA1 genes in the liver, muscle, and lipid tissues were compared between a high-cholesterol group and control group in mice. The results showed that ABCA1 was up-regulated by high cholesterol conditions in liver, muscle and lipid tissues. SREBP1C was up-regulated by high cholesterol conditions in the liver and lipid tissues and down-regulated by high cholesterol conditions in the muscle tissue. MicroRNA-33 and SREBP2 were down-regulated by high cholesterol conditions in the liver and muscle tissues and up-regulated by high cholesterol conditions in the lipid tissue. Our study suggests that antisense therapeutic targeting of microRNA-33 may be a potential biomarker for cardiovascular disease.
微小RNA是一类调控基因表达的短链非编码RNA。此前已鉴定出几种对冠状动脉疾病评估有用的微小RNA。微小RNA-33位于固醇调节元件结合蛋白（SREBP）的内含子中，控制胆固醇稳态。为了探究微小RNA-33作为高胆固醇疾病潜在生物标志物的可能性，我们通过给小鼠喂食高脂饮食建立了冠心病小鼠模型。比较了高胆固醇组和对照组小鼠肝脏、肌肉及脂质组织中微小RNA-33、SREBP和ATP结合盒转运蛋白A1（ABCA1）基因的表达差异。结果显示，在肝脏、肌肉及脂质组织中，高胆固醇条件使ABCA1上调。在肝脏和脂质组织中，高胆固醇条件使SREBP1C上调，而在肌肉组织中使其下调。在肝脏和肌肉组织中，高胆固醇条件使微小RNA-33和SREBP2下调，而在脂质组织中使其上调。我们的研究表明，针对微小RNA-33的反义治疗靶向可能是心血管疾病的潜在生物标志物。
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