Lü Xueqin, Wu Yaokang, Lin Lu, Xu Xianhao, Yu Wenwen, Cui Shixiu, Li Jianghua, Du Guocheng, Liu Long
Science Center for Future Foods, Jiangnan University, Wuxi 214122, Jiangsu, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2021 May 25;37(5):1619-1636. doi: 10.13345/j.cjb.200543.
As a typical food safety industrial model strain, Bacillus subtilis has been widely used in the field of metabolic engineering due to its non-pathogenicity, strong ability of extracellular protein secretion and no obvious codon preference. In recent years, with the rapid development of molecular biology and genetic engineering technology, a variety of research strategies and tools have been used to construct B. subtilis chassis cells for efficient synthesis of biological products. This review introduces the research progress of B. subtilis from the aspects of promoter engineering, gene editing, genetic circuit, cofactor engineering and pathway enzyme assembly. Then, we also summarized the application of B. subtilis in the production of biological products. Finally, the future research directions of B. subtilis are prospected.
枯草芽孢杆菌作为一种典型的食品安全工业模式菌株，因其无致病性、胞外蛋白分泌能力强且无明显密码子偏好性，已在代谢工程领域得到广泛应用。近年来，随着分子生物学和基因工程技术的快速发展，多种研究策略和工具被用于构建枯草芽孢杆菌底盘细胞以高效合成生物产品。本文综述了枯草芽孢杆菌在启动子工程、基因编辑、基因回路、辅因子工程和途径酶组装等方面的研究进展。此外，还总结了枯草芽孢杆菌在生物产品生产中的应用。最后，对枯草芽孢杆菌未来的研究方向进行了展望。
