School of Pharmaceutical Science, Liaoning University, Shenyang, Liaoning, P.R. China.
Anorectal Department, The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P.R. China.
Nat Prod Res. 2022 Jun;36(12):2977-2983. doi: 10.1080/14786419.2021.1935931. Epub 2021 Jun 4.
(Thunb.) Juss (), a perennial herb, is widely used as a medicinal plant in Chinese folk. This study aims to isolate and identify the chemical constituents from and evaluate their antioxidant activity. Normal silica column chromatography, ODS silica column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC methods were used to obtain a new compound named 3-n-pentadecyl-4'-methoxyluteolin (1) and two known compounds including luteolin-7-O-β-D-glucuronide methyl ester (2) and apigenin-7-O-β-D-glucuronide methyl ester (3). The antioxidant activity of these compounds was determined by DPPH and ABTS methods and the IC50 values were calculated. The IC50 values of ABTS scavenging activity of 1, 2 and 3 were 12.99 ± 0.09 μM, 7.13 ± 0.07 μM and 5.15 ± 0.08 μM, respectively, and the IC50 values of DPPH scavenging activity of 1, 2 and 3 were 51.86 ± 0.41 μM, 23.95 ± 0.71 μM and 25.06 ± 0.65 μM, respectively. All the compounds exhibited good antioxidant activities in vitro.
（Thunb.）Juss（），一种多年生草本植物，在中国民间广泛用作药用植物。本研究旨在从 中分离和鉴定化学成分，并评价其抗氧化活性。采用正相硅胶柱色谱、ODS 硅胶柱色谱、Sephadex LH-20 柱色谱和半制备 HPLC 方法，从 中获得了一种新化合物，命名为 3-n-十五烷基-4'-甲氧基木犀草素（1）和两种已知化合物，包括木犀草素-7-O-β-D-葡萄糖醛酸甲酯（2）和芹菜素-7-O-β-D-葡萄糖醛酸甲酯（3）。通过 DPPH 和 ABTS 方法测定了这些化合物的抗氧化活性，并计算了 IC50 值。化合物 1、2 和 3 的 ABTS 清除活性的 IC50 值分别为 12.99±0.09 μM、7.13±0.07 μM 和 5.15±0.08 μM，化合物 1、2 和 3 的 DPPH 清除活性的 IC50 值分别为 51.86±0.41 μM、23.95±0.71 μM 和 25.06±0.65 μM。所有化合物在体外均表现出良好的抗氧化活性。
