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分析和定量检测干果中的天然黄曲霉毒素 B1,并进行物理和生物解毒。

Analysis and quantification of naturally occurring aflatoxin B1 in dry fruits with subsequent physical and biological detoxification.

机构信息

Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan.

AgriPak Labs, Rawalpindi, Pakistan (Licensee Romer Labs Inc, USA).

出版信息

Nat Prod Res. 2022 Jun;36(12):3100-3104. doi: 10.1080/14786419.2021.1935930. Epub 2021 Jun 4.

Abstract

Current research involves extraction, identification and detoxification of mycotoxins from ten dry fruit samples. Mycotoxins were identified by high performance thin layer chromatography followed by physical and biological detoxification, analysed by HPLC. Three fungal species were observed after isolation including, and HP-TLC analysis revealed the presence of mycotoxin, aflatoxin B1 ranging from 0.000303-0.03636 mg/kg in all samples. Results were further analysed through various statistical tests. Detoxification methods proved to be cost effective and easily implementable. Concentration of aflatoxin B1 in pine nuts was reduced to 0.0043 mg/kg and 0.0039 mg/kg in dry dates through UV based detoxification. Solarisation reduced the concentration of aflatoxin B1 in figs to 0.0044 mg/kg. 90% aflatoxins were detoxified by UV treatment while powder detoxified 90% mycotoxin. This research concludes that the studied detoxification methods can be generalised on larger scale to benefit the dry fruit industry worldwide.

摘要

目前的研究涉及从十个干果样本中提取、鉴定和脱除霉菌毒素。采用高效薄层层析法对霉菌毒素进行鉴定,然后进行物理和生物解毒,再用 HPLC 进行分析。分离后观察到三种真菌,包括 、 。HP-TLC 分析显示,所有样品中均存在 0.000303-0.03636 mg/kg 的霉菌毒素黄曲霉毒素 B1。结果通过各种统计检验进一步分析。解毒方法被证明具有成本效益且易于实施。通过基于紫外线的解毒,松仁中的黄曲霉毒素 B1 浓度降低至 0.0043mg/kg 和 0.0039mg/kg,干枣中的黄曲霉毒素 B1 浓度降低至 0.0044mg/kg。太阳辐射将无花果中的黄曲霉毒素 B1 浓度降低至 0.0044mg/kg。紫外线处理可脱除 90%的黄曲霉毒素, 粉可脱除 90%的霉菌毒素。本研究得出结论,所研究的解毒方法可以在更大范围内推广,使全球干果产业受益。

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