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[通过目视筛选、薄层色谱法和酶联免疫吸附测定法测定无花果干中的黄曲霉毒素B1]

[Determination of aflatoxin B1 in dried figs by visual screening, thin-layer chromatography and ELISA].

作者信息

Reichert N, Steinmeyer S, Weber R

机构信息

Institut für Lebensmittelchemie, Technische Universität Berlin.

出版信息

Z Lebensm Unters Forsch. 1988 Jun;186(6):505-8.

PMID:3136605
Abstract

Aflatoxin B1-contaminated fruits were sorted out from 250 kg dried figs (five Turkish and three Greek batches) by bright-greenish-yellow fluorescence under UV light. The aflatoxins of the fluorescent figs were extracted by simple soaking in methanol. Aflatoxin B1 was determined by thin-layer chromatography. Parallel to this, an extraction for the determination of aflatoxin B1 was developed by a competitive ELISA and the two methods were compared with each other. In a highly contaminated batch of Turkish figs, statistically there was one fig among 350 which had a high aflatoxin content (greater than 100 ng/g fig) and one fig amongst 140 fruits with an aflatoxin B1 content of greater than 10 ng B1/g fig.

摘要

在紫外光下,通过亮黄绿色荧光从250千克干无花果(5批土耳其产和3批希腊产)中挑选出被黄曲霉毒素B1污染的果实。对有荧光的无花果中的黄曲霉毒素进行甲醇简单浸泡提取。采用薄层色谱法测定黄曲霉毒素B1。与此同时,开发了一种竞争性酶联免疫吸附测定法用于黄曲霉毒素B1的提取,并将这两种方法进行了相互比较。在一批污染严重的土耳其无花果中,从统计学角度来看,350个无花果中有1个黄曲霉毒素含量很高(大于100纳克/克无花果),140个果实中有1个黄曲霉毒素B1含量大于10纳克B1/克无花果。

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