Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery College, Huazhong Agricultural University, Wuhan430070, People's Republic of China.
Br J Nutr. 2022 Feb 28;127(4):490-502. doi: 10.1017/S000711452100194X. Epub 2021 Jun 4.
The study was conducted to determine the effects of three dietary Se sources, such as sodium-selenite (S-S), seleno-yeast (S-Y) and seleno-methionine (S-M), on Se concentration, glutathione peroxidase (GPX) and TXNRD activities, and mRNA expression of fifteen representative selenoproteins, and protein expression of four endoplasmic reticulum-resided selenoproteins in a wide range of tissues of yellow catfish. Compared with S-S and S-M groups, dietary S-Y significantly decreased growth performance and feed utilisation of yellow catfish. Dietary Se sources significantly influenced Se contents in the spleen, dorsal muscle and the kidney, GPX activities in spleen, kidney, intestine, muscle and mesenteric fat, and TXNRD activities in the heart, intestine and mesenteric fat. Among ten tested tissues, dietary Se sources influenced mRNA expression of GPX4 and SELENOK in three tissues; GPX3, SELENOS and TXNRD2 in four tissues; SELENOF, SELENON and DIO2 in five tissues; SELENOM, GPX1/2 and TXNRD3 in six tissues; SELENOW in seven tissue and SELENOP and SELENOT in eight tissues. Based on these observations above, S-S and S-M seem to be suitable Se sources for improving growth performance and feed utilisation of yellow catfish. Dietary Se sources differentially influence the expression of selenoproteins in various tissues of yellow catfish. For the first time, we determined the expression of selenoproteins in fish in responses to dietary Se sources, which contributes to a better understanding of the functions and regulatory mechanisms of selenoporteins.
本研究旨在确定三种膳食硒源（亚硒酸钠[S-S]、酵母硒[S-Y]和硒代蛋氨酸[S-M]）对黄颡鱼各组织硒浓度、谷胱甘肽过氧化物酶（GPX）和硫氧还蛋白还原酶（TXNRD）活性以及 15 种代表性硒蛋白的 mRNA 表达和 4 种内质网驻留硒蛋白的蛋白表达的影响。与 S-S 和 S-M 组相比，饲粮 S-Y 显著降低了黄颡鱼的生长性能和饲料利用率。饲粮硒源显著影响了黄颡鱼脾脏、背肌和肾脏中的硒含量、脾脏、肾脏、肠道、肌肉和肠系膜脂肪中的 GPX 活性以及心脏、肠道和肠系膜脂肪中的 TXNRD 活性。在测试的 10 种组织中，饲粮硒源影响了 3 种组织中 GPX4 和 SELENOK 的 mRNA 表达、4 种组织中 GPX3、SELENOS 和 TXNRD2 的 mRNA 表达、5 种组织中 SELENOF、SELENON 和 DIO2 的 mRNA 表达、6 种组织中 SELENOM、GPX1/2 和 TXNRD3 的 mRNA 表达、7 种组织中 SELENOW 的 mRNA 表达和 8 种组织中 SELENOP 和 SELENOT 的 mRNA 表达。基于以上观察结果，S-S 和 S-M 似乎是提高黄颡鱼生长性能和饲料利用率的适宜硒源。饲粮硒源对黄颡鱼各组织中硒蛋白的表达有不同的影响。本研究首次测定了鱼类对饲粮硒源的反应中硒蛋白的表达，有助于更好地理解硒蛋白的功能和调控机制。
