Hubei Hongshan Laboratory, Fishery College, Huazhong Agriculture University, Wuhan 430070, China.
Hubei Hongshan Laboratory, Fishery College, Huazhong Agriculture University, Wuhan 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Gene. 2023 Jul 15;873:147461. doi: 10.1016/j.gene.2023.147461. Epub 2023 May 4.
The selenok, selenot and selenop are three key selenoproteins involved in stress response. Our study, using the yellow catfish Pelteobagrus fulvidraco as the experimental animal, obtained the 1993-bp, 2000-bp and 1959-bp sequences of selenok, selenot and selenop promoters, respectively, and predicted the binding sites of several transcriptional factors on their promoters, such as Forkhead box O 4 (FoxO4), activating transcription factor 4 (ATF4), Kruppel-like factor 4 (KLF4) and nuclear factor erythroid 2-related factor 2 (NRF2). Selenium (Se) increased the activities of the selenok, selenot and selenop promoters. FoxO4 and Nrf2 can directly bind with selenok promoter and controlled selenok promoter activities positively; KLF4 and Nrf2 can directly bind with selenot promoter and controlled selenot promoter activities positively; FoxO4 and ATF4 can directly bind to selenop promoter and regulated selenop promoter activities positively. Se promoted FoxO4 and Nrf2 binding to selenok promoter, KLF4 and Nrf2 binding to selenot promoter, and FoxO4 and ATF4 binding to selenop promoter. Thus, we provide the first evidence for FoxO4 and Nrf2 bindnig elements in selenok promoter, KLF4 and Nrf2 binding elements in selenot promoter, and FoxO4 and ATF4 binding elements in selenop promoter, and offer novel insight into regulatory mechanism of these selenoproteins induced by Se.
硒代、硒诺和硒蛋白是三种参与应激反应的关键硒蛋白。本研究以黄颡鱼（Pelteobagrus fulvidraco）为实验动物，获得了 selenok、selenot 和 selenop 启动子的 1993bp、2000bp 和 1959bp 序列，并预测了它们启动子上几种转录因子的结合位点，如 Forkhead box O4（FoxO4）、激活转录因子 4（ATF4）、Kruppel-like factor 4（KLF4）和核因子红细胞 2 相关因子 2（NRF2）。硒（Se）增加了 selenok、selenot 和 selenop 启动子的活性。FoxO4 和 Nrf2 可以直接与 selenok 启动子结合，并正向控制 selenok 启动子活性；KLF4 和 Nrf2 可以直接与 selenot 启动子结合，并正向控制 selenot 启动子活性；FoxO4 和 ATF4 可以直接与 selenop 启动子结合，并正向调节 selenop 启动子活性。Se 促进 FoxO4 和 Nrf2 与 selenok 启动子结合，KLF4 和 Nrf2 与 selenot 启动子结合，以及 FoxO4 和 ATF4 与 selenop 启动子结合。因此，我们首次提供了 FoxO4 和 Nrf2 结合 selenok 启动子的结合元件、KLF4 和 Nrf2 结合 selenot 启动子的结合元件，以及 FoxO4 和 ATF4 结合 selenop 启动子的结合元件的证据，并为 Se 诱导这些硒蛋白的调控机制提供了新的见解。
