Department of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, United States.
Department of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, United States.
Environ Pollut. 2021 Sep 15;285:117480. doi: 10.1016/j.envpol.2021.117480. Epub 2021 May 29.
Antibiotic resistance genes (ARGs) may be introduced to agricultural soil through the land application of cattle manure. During a rainfall event, manure-borne ARGs may infiltrate into subsurface soil and leach into groundwater. The objective of this study was to characterize and model the vertical transport of manure-borne ARGs through soil following the land application of beef cattle manure on soil surface. In this study, soil column experiments were conducted to evaluate the influence of manure application on subsurface transport of four ARGs: erm(C), erm(F), tet(O) and tet(Q). An attachment-detachment model with the decay of ARGs in the soil was used to simulate the breakthrough of ARGs in leachates from the control column (without manure) and treatment (with manure) soil columns. Results showed that the first-order attachment coefficient (k) was five to six orders of magnitude higher in the treatment column than in the control column. Conversely, the first-order detachment and decay coefficients (k and μ) were not significantly changed due to manure application. These findings suggest that in areas where manure is land-applied, some manure-borne bacteria-associated ARGs will be attached to the soil, instead of leaching to groundwater in near terms.
抗生素耐药基因（ARGs）可能通过牛粪的土地施用而引入农业土壤。在降雨事件中，粪源 ARGs 可能渗透到地下土壤并渗入地下水。本研究的目的是描述和模拟在牛粪施用于土壤表面后，粪源 ARGs 通过土壤的垂直迁移。在这项研究中，进行了土壤柱实验，以评估施粪对四种 ARGs（erm(C)、erm(F)、tet(O)和 tet(Q)）在地下迁移的影响。采用带有 ARGs 在土壤中衰减的附着-脱附模型来模拟对照柱（无粪）和处理（施粪）土壤柱中 ARGs 在淋出液中的突破。结果表明，处理柱中的一阶附着系数（k）比对照柱高五到六个数量级。相反，由于粪肥的施用，一阶脱附和衰减系数（k 和 μ）并没有显著变化。这些发现表明，在施用粪肥的地区，一些粪源细菌相关的 ARGs 将附着在土壤上，而不是在短期内渗入地下水。
