State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China; College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.
State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Vet Microbiol. 2021 Aug;259:109134. doi: 10.1016/j.vetmic.2021.109134. Epub 2021 May 26.
Increasing evidence shows that gut microbiota plays a critical role in host immune system development and immune regulation, thus the composition of gut microbiota may affect how individuals respond to immunizations. Currently, little evidence is available on the correlation between porcine gut microbiota and vaccine immune response. Here, we investigated the influence of gut microbiota on immune response in pigs to porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. Based on the antibody levels for PRRSV, the immunized pigs were divided into three groups (high, low, and others), and followed by virulent PRRSV challenge. The comprehensive analysis of microbial composition revealed that gut microbiota was similar in the richness and diversity among different groups before immunization. After immunization, the richness and diversity of gut microbial community in the high group were still similar to the low group, although there was a decrease in community diversity overtime. Interestingly, the antibody titer was positively correlated with the abundance of Lactobacillus in gut microbiota in immunized pigs. Further analysis indicated that gut microbial composition might be correlated to the clinical parameters such as body weight and rectal temperature after virus challenge. Taken together, our findings suggest that certain specific members of gut microbiota, such as Lactobacillus may serve as a mechanism for regulating the immune response following immunization in pigs.
越来越多的证据表明，肠道微生物群在宿主免疫系统发育和免疫调节中起着关键作用，因此肠道微生物群的组成可能会影响个体对免疫接种的反应。目前，关于猪肠道微生物群与疫苗免疫反应之间的相关性的证据很少。在这里，我们研究了肠道微生物群对猪对猪繁殖与呼吸综合征病毒（PRRSV）疫苗免疫反应的影响。基于针对 PRRSV 的抗体水平，将免疫接种的猪分为三组（高、低和其他），然后进行强毒 PRRSV 攻毒。微生物组成的综合分析表明，在免疫接种前，不同组之间的肠道微生物丰富度和多样性相似。免疫接种后，高组的肠道微生物群落丰富度和多样性仍与低组相似，尽管随着时间的推移，群落多样性有所下降。有趣的是，抗体滴度与免疫猪肠道微生物群中乳杆菌的丰度呈正相关。进一步分析表明，肠道微生物组成可能与病毒攻毒后的体重和直肠温度等临床参数相关。总之，我们的研究结果表明，肠道微生物群中的某些特定成员，如乳杆菌，可能是调节猪免疫接种后免疫反应的机制之一。
