Jiangxi Key Laboratory of Nanobiomaterials, Institute of Advanced Materials, East China Jiaotong University, Nanchang 330013, People's Republic of China.
Key Laboratory for Anisotropy and Texture of Materials (ATM), Ministry of Education (MoE), School of Material Science and Engineering, Northeastern University, Shenyang 110819, People's Republic of China.
Biomed Mater. 2021 Jun 18;16(4). doi: 10.1088/1748-605X/ac0863.
Macrophages play a central role in the host response and the integration of implant materials. The nanostructured TiF/TiOcoating (FOTi) on titanium surfaces has shown multiple properties, including antibacterial properties and bioactivity. However, little is known about the effects of these coatings on the regulation of macrophage activity and the subsequent immunomodulatory effects on osteogenesis. In this study, the behavior of macrophages on the FOTi samples was evaluated, and conditioned medium was collected and used to stimulate MC3T3-E1 cells. The results showed that the FOTi samples stimulated macrophage elongation and promoted the production of proinflammatory cytokines at 24 h, while induced macrophage polarization to the anti-inflammatory M2 phenotype at 72 h. Furthermore, the immune microenvironment generated by macrophage/ FOTi samples interactions effectively promoted the osteogenic differentiation of MC3T3-E1 cells, as evidenced by improved cell adhesion, enhanced alkaline phosphatase activity and extracellular matrix mineralization, and upregulated osteogenesis-related gene expression. In summary, the FOTi samples mediated macrophage phenotype behaviors and induced beneficial immunomodulatory effects on osteogenesis, which could be a potential strategy for the surface modification of bone biomaterials.
巨噬细胞在宿主反应和植入材料的整合中起着核心作用。钛表面的纳米结构 TiF/TiO 涂层（FOTi）具有多种特性，包括抗菌性能和生物活性。然而，对于这些涂层对巨噬细胞活性的调节以及对成骨的随后免疫调节作用知之甚少。在这项研究中，评估了巨噬细胞在 FOTi 样品上的行为，并收集了条件培养基用于刺激 MC3T3-E1 细胞。结果表明，FOTi 样品在 24 小时刺激巨噬细胞伸长，并促进促炎细胞因子的产生，而在 72 小时诱导巨噬细胞向抗炎 M2 表型极化。此外，巨噬细胞/FOTi 样品相互作用产生的免疫微环境有效促进了 MC3T3-E1 细胞的成骨分化，表现为细胞黏附增强、碱性磷酸酶活性和细胞外基质矿化增强以及成骨相关基因表达上调。总之，FOTi 样品介导了巨噬细胞表型行为，并对成骨诱导了有益的免疫调节作用，这可能是骨生物材料表面改性的一种潜在策略。
