噬菌体λ在大肠杆菌宿主突变体ER437中的阻遏物和int合成。

Repressor and int synthesis of bacteriophage lambda in the E. coli host mutant ER437.

作者信息

Belfort M, Kass N, Oppenheim A, Katzir N, Oppenheim A B

出版信息

Mol Gen Genet. 1977 Oct 24;155(3):347-9. doi: 10.1007/BF00272815.

Abstract

Analysis of lambda phage infection of the host mutant ER437 by SDS polyacrylamide gel electrophoresis and autoradiography has revealed altered expression of repressor and integration function (Int). We show that in this host Int as well as repressor synthesis is not dependent upon the lambdacIII gene product in the usual manner, nor is their synthesis turned off in the normal way.

摘要

通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和放射自显影分析λ噬菌体对宿主突变体ER437的感染，揭示了阻遏物和整合功能（Int）表达的改变。我们发现，在这种宿主中，Int以及阻遏物的合成并不以通常的方式依赖于λcIII基因产物，它们的合成也不会以正常方式被关闭。