Oppenheim A, Oppenheim A B
Mol Gen Genet. 1978 Sep 20;165(1):39-46. doi: 10.1007/BF00270374.
The activation of the int gene by the cII and cIII gene products was studied by analysing int expression following infection of UV-irradiated cells by various phage mutants. Residual expression of int, probably from Pl, takes place in the absence of cII/cIII activation. Activation of the int gene, like that of the cI repressor gene, is poor at low multiplicities of infection. The mutation intC, which allows constitutive int expression in the lysogenic state, partially relieves the requirement for cII and cIII activation. The kinetics of Int synthesis after addition of the inhibitor rifampicin suggest that the activation occurs at the transcriptional level.
通过分析各种噬菌体突变体感染紫外线照射细胞后的 int 表达，研究了 cII 和 cIII 基因产物对 int 基因的激活作用。在没有 cII/cIII 激活的情况下，int 可能从 P1 启动子开始有残留表达。与 cI 阻遏基因一样，在低感染复数时 int 基因的激活效果不佳。intC 突变允许在溶原状态下组成型表达 int，部分缓解了对 cII 和 cIII 激活的需求。添加抑制剂利福平后 Int 合成的动力学表明激活发生在转录水平。
