Department of Pulmonary, Cukurova University School of Medicine, Adana 01330, Turkey; Department of Translational Medicine, Institute of Health Sciences, Cukurova University, Adana, Turkey; Department of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, Adana, Turkey.
Department of Biotechnology, Institute of Natural and Applied Sciences, Cukurova University, Adana, Turkey.
Cancer Treat Res Commun. 2021;28:100406. doi: 10.1016/j.ctarc.2021.100406. Epub 2021 May 25.
Covid-19 Pneumonia of SARS-CoV-2 pandemic infection, persists to have high disease burden especially in cancer patients. Increased inflammation and thromboembolic processes are blamed to influence cancer patients more than the others but due to lack of knowledge regarding the pathophysiology of the both the virus itself and the response of the host, more basic and translational disease modeling research is needed to understand Cancer-Covid-19 interaction. In this study, serum samples from the patients, who were hospitalized due to Covid-19 pneumonia, applied to different cancer cells and cytotoxicity, motility, proliferation and gene expression analysis were performed. Serum samples derived from healthy volunteers and the fetal bovine serum that is used regularly in cell culture experiments used as controls. Hospitalized Covid-19 patients who had also cancer, were retrospectively screened, and their clinical course were recorded. Overall 12 Patient (PS) and 4 healthy serums (CS) were included in the experiments. PS applied cells showed increased motility in A549 cells as well as lost cell to cell connection in MCF7 and HCT116 cells, and induced expression of VIM, ZEB1 and SNAIL2 mRNA levels. Eight cancer diagnosed patients who were hospitalized due to Covid-19 between April and September 2020 were also reviewed retrospectively, which 5 of them were dead during SARS-CoV-2 infection. Thorax CT images of the 2 patients showed increased metastatic nodules in the lungs as of January 2021. The results of the study indicate that metastasis may be one of the prolonged consequences of COVID-19 pandemic in cancer sufferers.
SARS-CoV-2 大流行感染引起的新冠病毒肺炎仍然存在高疾病负担，尤其是在癌症患者中。炎症和血栓栓塞过程增加被认为比其他因素更能影响癌症患者，但由于对病毒本身和宿主反应的病理生理学缺乏了解，需要更多基础和转化疾病建模研究来理解癌症-新冠病毒相互作用。在这项研究中，将因新冠病毒肺炎住院的患者的血清样本应用于不同的癌细胞，并进行细胞毒性、运动性、增殖和基因表达分析。将来自健康志愿者的血清样本和细胞培养实验中常规使用的胎牛血清作为对照。回顾性筛选因新冠病毒肺炎住院且患有癌症的患者，并记录其临床病程。实验共纳入 12 名患者（PS）和 4 名健康供者（CS）的血清样本。PS 应用的细胞在 A549 细胞中表现出运动性增加，在 MCF7 和 HCT116 细胞中失去细胞间连接，并诱导 VIM、ZEB1 和 SNAIL2 mRNA 水平的表达。还回顾性分析了 2020 年 4 月至 9 月期间因新冠病毒肺炎住院的 8 名癌症患者，其中 5 名在 SARS-CoV-2 感染期间死亡。2 名患者的胸部 CT 图像显示 2021 年 1 月肺部转移性结节增加。研究结果表明，转移可能是新冠病毒肺炎大流行在癌症患者中遗留的长期后果之一。
