Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China.
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Sichuan Province, Southwest Minzu University, Chengdu, China.
BMC Genomics. 2021 Jun 5;22(1):417. doi: 10.1186/s12864-021-07730-y.
Intramuscular adipocytes differentiation is a complex process, which is regulated by various transcription factor, protein factor regulators and signal transduction pathways. However, the proteins and signal pathways that regulates goat intramuscular adipocytes differentiation remains unclear.
In this study, based on nanoscale liquid chromatography mass spectrometry analysis (LC-MS/MS), the tandem mass tag (TMT) labeling analysis was used to investigate the differentially abundant proteins (DAPs) related with the differentiation process of goat intramuscular adipocytes. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes enrichment and protein-protein interaction network analyses were performed for the characterization of the identified DAPs. The candidate proteins were verified by parallel reaction monitoring analysis. As a result, a total of 123 proteins, 70 upregulation proteins and 53 downregulation proteins, were identified as DAPs which may be related with the differentiation process of goat intramuscular adipocytes. Furthermore, the cholesterol metabolism pathway, glucagon signaling pathway and glycolysis / gluconeogenesis pathway were noticed that may be the important signal pathways for goat Intramuscular adipocytes differentiation.
By proteomic comparison between goat intramuscular preadipocytes (P_IMA) and intramuscular adipocytes (IMA), we identified a series protein that might play important role in the goat intramuscular fat differentiation, such as SRSF10, CSRP3, APOH, PPP3R1, CRTC2, FOS, SERPINE1 and AIF1L, could serve as candidates for further elucidate the molecular mechanism of IMF differentiation in goats.
肌内脂肪细胞分化是一个复杂的过程，受多种转录因子、蛋白因子调节剂和信号转导途径的调控。然而，调控山羊肌内脂肪细胞分化的蛋白质和信号通路尚不清楚。
本研究基于纳升液相色谱-串联质谱分析（LC-MS/MS），采用串联质量标签（TMT）标记分析技术，研究了与山羊肌内脂肪细胞分化过程相关的差异丰度蛋白（DAPs）。对鉴定出的 DAPs 进行基因本体论（GO）、京都基因与基因组百科全书（KEGG）富集分析和蛋白质-蛋白质相互作用网络分析，以描述其特征。通过平行反应监测分析对候选蛋白进行验证。结果共鉴定出 123 种差异表达蛋白，其中 70 种上调蛋白和 53 种下调蛋白，这些蛋白可能与山羊肌内脂肪细胞的分化过程有关。此外，还注意到胆固醇代谢途径、胰高血糖素信号通路和糖酵解/糖异生途径可能是山羊肌内脂肪细胞分化的重要信号通路。
通过对山羊肌内前脂肪细胞（P_IMA）和肌内脂肪细胞（IMA）的蛋白质组比较，我们鉴定出了一系列可能在山羊肌内脂肪分化中起重要作用的蛋白质，如 SRSF10、CSRP3、APOH、PPP3R1、CRTC2、FOS、SERPINE1 和 AIF1L，它们可以作为进一步阐明山羊 IMF 分化分子机制的候选基因。
