Molecular and expression characteristics of resistin (RETN) and its effects on the differentiation of intramuscular preadipocyte in goat.

is a hormone secreted by adipocytes, which plays an important role in glucose and lipid metabolism. The aim of this study is to clone and obtain the full length open reading frame (ORF) of goat gene sequence, and to reveal its molecular and expression characteristics. Simultaneously, we explore its effect on the differentiation of intramuscular preadipocytes in goat. The full length ORF sequence of goat gene was cloned by RT-PCR technique, and bioinformatics analysis was performed though relevant biological softwares. In this study, the expression of mRNA in goat tissues and intramuscular preadipocytes during differentiation was detected by qPCR technique. Furthermore, RNA interference was used to explore the effects of on intramuscular preadipocytes differentiation in goat. The results showed that the cloned goat gene sequence was 428 bp in length, of which the ORF was 330 bp, encoding 109 amino acids. Sequence analysis revealed that it had 12 phosphorylation sites and an O-glycosylation site, and its protein contained a signal peptide sequence. Also, the gene is expressed in goat various analyzed tissues, and the results showed that the expression of gene in lung tissue was higher than that in other analyzed tissues of goat ( < .01). Moreover, the expression level of gene in the goat's intramuscular preadipocytes decreased first and then increased, and reached the highest on the fifth day, which was significantly higher than that of undifferentiated intramuscular preadipocytes ( < .001). After transfecting intramuscular preadipocyte with siRNA, we found that the mRNA level of was significantly down-regulated by 70% and 87% ( < .01). Oil red O staining results showed that the interference of gene can promote the differentiation of intramuscular preadipocytes. After knockdown of with siRNA, the and 1 genes were significantly up-regulated ( < .01). Thus, it can be inferred that inhibits the differentiation of goat intramuscular preadipocytes, probably through regulating the expression of 2 and -1 genes.