Rosenfeld P, Sutanto W, Levine S, De Kloet E R
Rudolf Magnus Institute for Pharmacology, Medical Faculty, University of Utrecht, The Netherlands.
Brain Res. 1988 Jul 1;470(1):113-8. doi: 10.1016/0165-3806(88)90206-4.
The ontogeny of the corticoid receptors in the rat hippocampus was examined by in vitro [3H]corticosterone (CORT) binding to soluble molecules in the cytosol, using the selective Type II glucocorticoid agonist, RU 28362, to discriminate between Type I and Type II receptor sites. Type I receptors were undetectable until 8 days after birth. From this age on, the receptor showed adult characteristics for both the binding capacity (Bmax) and affinity (Kd). The Type II receptor concentration increased gradually over the observed period; however, at 3 weeks of age concentrations were still only about 65% those found in adults. The binding affinity of Type II to CORT was high during the first week of life but decreased thereafter towards adult value. These data thus suggest clear distinctions in the developmental patterns of Type I and Type II receptors for corticosteroids in the rat.
通过体外[3H]皮质酮（CORT）与胞浆中可溶性分子的结合，使用选择性II型糖皮质激素激动剂RU 28362来区分I型和II型受体位点，研究了大鼠海马中皮质激素受体的个体发生。出生后8天之前未检测到I型受体。从这个年龄开始，受体在结合能力（Bmax）和亲和力（Kd）方面都表现出成年特征。II型受体浓度在观察期内逐渐增加；然而，在3周龄时，其浓度仍仅约为成年大鼠的65%。II型受体对CORT的结合亲和力在出生后的第一周较高，但此后逐渐下降至成年水平。因此，这些数据表明大鼠中I型和II型糖皮质激素受体的发育模式存在明显差异。
