Sarrieau A, Sharma S, Meaney M J
Douglas Hospital Research Centre, Department of Psychiatry, Faculty of Medicine, McGill University, Montreal, Que., Canada.
Brain Res. 1988 Sep 1;471(1):158-62. doi: 10.1016/0165-3806(88)90162-9.
We report here on developmental changes in [3H]RU 28362 (glucocorticoid receptor) and [3H]aldosterone (mineralocorticoid receptor) binding capacity in soluble fractions prepared from hippocampal tissue. [3H]RU 28362 binding was low on Day 3 of life (less than 30% of that observed in adults) and increased towards adult values during the second and third weeks of life, a pattern virtually identical to that previously reported for dexamethasone binding. In contrast, [3H]aldosterone binding on Day 3 of life was only slightly lower than that observed in adults and reached adult values by Day 7. Postnatal handling of rat pups, which has been shown to increase dexamethasone binding in hippocampus, resulted in a significant increase in [3H]RU 28362 binding capacity in hippocampus, but had no effect on [3H]aldosterone binding.
我们在此报告从海马组织制备的可溶性组分中[3H]RU 28362（糖皮质激素受体）和[3H]醛固酮（盐皮质激素受体）结合能力的发育变化。出生后第3天，[3H]RU 28362结合水平较低（不到成年动物的30%），在出生后第二和第三周逐渐升高至成年水平，这一模式与先前报道的地塞米松结合模式几乎相同。相比之下，出生后第3天的[3H]醛固酮结合仅略低于成年动物，到第7天达到成年水平。已证明对新生大鼠幼崽的产后处理可增加海马中的地塞米松结合，这导致海马中[3H]RU 28362结合能力显著增加，但对[3H]醛固酮结合无影响。
