Prithika Udayakumar, Balamurugan Krishnaswamy
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
Microbes Infect. 2021 Nov-Dec;23(9-10):104846. doi: 10.1016/j.micinf.2021.104846. Epub 2021 Jun 4.
Routinely, studies were performed using age-synchronized group of Caenorhabditis elegans as host which suggested a collective response by the host system. Here, we report the modulation of immune response in a single nematode against Staphylococcus aureus and Proteus mirabilis. Initially, the survival of wild-type N2 was tested and was found that S. aureus killed single nematode at 42 h while P. mirabilis failed to provoke infection but colonized the nematode's intestine. With this milieu, the pathogenicity of the bacteria was assessed by Fourier Transform Infra-Red (FTIR) spectroscopy and Cyclic Voltammetry (CV) and was found that S. aureus in the presence of host elicited its virulence while P. mirabilis and Escherichia coli OP50 did not show any alteration. Vertical transmission of infection was also deduced by colony forming unit assay using Cyanine dyes. The MALDI-TOF/TOF analysis was also performed to identify the proteome changes in the single nematode that showcased different proteins related to various immune pathways. This study suggested the importance of understanding the infection pathology and traits of individual nematode which could help our understanding on otherwise the disordered processes during host and microbe interactions.
通常，研究使用年龄同步的秀丽隐杆线虫群体作为宿主进行，这表明宿主系统存在集体反应。在此，我们报告了单个线虫对金黄色葡萄球菌和奇异变形杆菌免疫反应的调节。最初，对野生型N2的存活率进行了测试，发现金黄色葡萄球菌在42小时时杀死单个线虫，而奇异变形杆菌未能引发感染，但定植在线虫的肠道中。在此环境下，通过傅里叶变换红外光谱（FTIR）和循环伏安法（CV）评估了细菌的致病性，发现宿主存在时金黄色葡萄球菌引发了其毒力，而奇异变形杆菌和大肠杆菌OP50没有表现出任何变化。还通过使用花青染料的菌落形成单位测定法推断了感染的垂直传播。还进行了基质辅助激光解吸电离飞行时间/飞行时间（MALDI-TOF/TOF）分析，以鉴定单个线虫中的蛋白质组变化，这些变化展示了与各种免疫途径相关的不同蛋白质。这项研究表明了解单个线虫的感染病理学和特征的重要性，这有助于我们理解宿主与微生物相互作用过程中原本无序的过程。
