过表达的circRANBP17作为一种癌基因，通过miR-635/RUNX2轴促进鼻咽癌。
Overexpressed circRANBP17 acts as an oncogene to facilitate nasopharyngeal carcinoma via the miR-635/RUNX2 axis.
作者信息
Zhou Minghui, Zhang Puwen, Zhao Yulin, Liu Rui, Zhang Yujie
机构信息
Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Department of Otorhinolaryngology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
出版信息
J Cancer. 2021 May 19;12(14):4322-4331. doi: 10.7150/jca.55794. eCollection 2021.
Circular RNAs (circRNAs) are implicated in the initiation and progress of several diseases, including cancer. However, the precise role of circRNAs in human nasopharyngeal carcinoma (NPC) remains unclear. In this research, we found a new circRNA hsa_circ_0001554 (circRANBP17), which was derived from the RAN binding protein 17 (RANBP17). Our qRT-PCR data found that circRANBP17 expression was up-regulated in NPC tissue and cells. Functional silencing studies revealed that circRANBP17 inhibited NPC cell proliferation and invasion and circRANBP17 down-regulation also reduced tumor growth in nude mice. MiR-635 was demonstrated as a direct target of circRANBP17; circRANBP17 up-regulated RUNX2 expression levels by sponging miR-635, thereby promoting NPC proliferation and invasion. Thus, our data provide the evidence for the first time that circRANBP17 is a new onco-circRNA via miR-635/RUNX2 axis regulation, and may function as a novel therapeutic target for NPC treatment.
环状RNA（circRNAs）与包括癌症在内的多种疾病的发生和发展有关。然而，circRNAs在人类鼻咽癌（NPC）中的具体作用仍不清楚。在本研究中，我们发现了一种新的环状RNA hsa_circ_0001554（circRANBP17），它来源于RAN结合蛋白17（RANBP17）。我们的qRT-PCR数据发现，circRANBP17在NPC组织和细胞中表达上调。功能沉默研究表明，circRANBP17抑制NPC细胞增殖和侵袭，circRANBP17下调也可减少裸鼠肿瘤生长。miR-635被证明是circRANBP17的直接靶点；circRANBP17通过海绵吸附miR-635上调RUNX2表达水平，从而促进NPC增殖和侵袭。因此，我们的数据首次证明circRANBP17通过miR-635/RUNX2轴调控是一种新的致癌环状RNA，并可能作为NPC治疗的新靶点发挥作用。
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