Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan, China
J Investig Med. 2020 Dec;68(8):1357-1363. doi: 10.1136/jim-2020-001438. Epub 2020 Aug 4.
Accumulating studies have shown that the dysregulation of microRNAs is related to the carcinogenesis and development of gastric cancer (GC), and the role of miR-635 in GC remains largely unknown. miR-635 and (KIFC1) mRNA expression in GC tissues and paracancerous tissues and cells were detected by quantitative real-time PCR. KIFC1 protein expression in GC tissues and paracancerous normal tissues and cells was detected by immunohistochemistry and western blot. Cell proliferation was monitored by Cell Counting Kit-8 assay and 5-bromo-2'-deoxyuridine assay. Transwell assay was employed to detect the migration and invasion of GC cells. The dual-luciferase reporter gene assay was adopted to detect the targeting relationship between miR-635 and KIFC1. Compared with paracancerous tissues, miR-635 expression was remarkably decreased in GC tissues; conversely, KIFC1 expression was significantly increased. Compared with human normal gastric epithelial cell GSE-1, miR-635 expression was markedly decreased in GC cell lines. Meanwhile, KIFC1 expression was significantly increased, and the Kaplan-Meier Plotter database showed that its high expression was remarkably associated with poor prognosis. Additionally, miR-635 can negatively regulate KIFC1. miR-635 can target KIFC1 to inhibit proliferation, migration and invasion of GC cells. Collectively, miR-635 is lowly expressed in GC, and it inhibits proliferation, migration and invasion of GC cells via regulating KIFC1.
越来越多的研究表明，miRNA 的失调与胃癌（GC）的发生发展有关，miR-635 在 GC 中的作用仍知之甚少。通过实时定量 PCR 检测 GC 组织和癌旁组织及细胞中 miR-635 和 （KIFC1）mRNA 的表达。通过免疫组织化学和 Western blot 检测 GC 组织和癌旁正常组织及细胞中 KIFC1 蛋白的表达。通过细胞计数试剂盒-8 检测和 5-溴-2'-脱氧尿苷检测监测细胞增殖。通过 Transwell 检测 GC 细胞的迁移和侵袭。采用双荧光素酶报告基因检测 miR-635 与 KIFC1 之间的靶向关系。与癌旁组织相比，GC 组织中 miR-635 的表达明显降低；相反，KIFC1 的表达显著增加。与人类正常胃上皮细胞 GSE-1 相比，GC 细胞系中 miR-635 的表达明显降低。同时，KIFC1 的表达显著增加，Kaplan-Meier Plotter 数据库显示其高表达与预后不良显著相关。此外，miR-635 可以负调控 KIFC1。miR-635 可以通过调节 KIFC1 来抑制 GC 细胞的增殖、迁移和侵袭。总之，miR-635 在 GC 中表达较低，通过调节 KIFC1 抑制 GC 细胞的增殖、迁移和侵袭。
