Department of Otology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Department of Otorhinolaryngology, Weihai Central Hospital, Weihai 264400, Shandong, China.
Aging (Albany NY). 2020 Oct 20;12(20):20645-20657. doi: 10.18632/aging.103963.
Accumulating lines of evidence indicate that circular RNAs (circRNAs) are involved in the pathogenesis of human cancers, including nasopharyngeal carcinoma (NPC). However, the influences of hsa_circ_0081534 upon the pathogenesis and dynamics of NPC are undescribed. In this study, we identified a circRNA hsa_circ_0081534 was significantly upregulated in NPC tissues and cell lines. Inhibition of hsa_circ_0081534 induced a decrease in NPC cells proliferation and invasion in vitro, and repressed tumor growth in vivo. In mechanism, hsa_circ_0081534 promoted NPC progression by sponging miR-508-5p. Fibronectin 1 (FN1) is a target gene of miR-508-5p. In addition, rescue assays showed that FN1 overexpression (or miR-508-5p inhibitors) abolished the roles of hsa_circ_0081534 inhibition on NPC cells proliferation and invasion. Therefore, hsa_circ_0081534 promoted the proliferation, and invasion of NPC cells via regulating the miR-508-5p/FN1 axis. Our findings suggested that hsa_circ_0081534 could be a novel therapeutic target for the treatment of NPC patients.
越来越多的证据表明，环状 RNA（circRNA）参与了人类癌症的发病机制，包括鼻咽癌（NPC）。然而，hsa_circ_0081534 对 NPC 发病机制和动态的影响尚未描述。在本研究中，我们鉴定出 hsa_circ_0081534 在 NPC 组织和细胞系中显著上调。抑制 hsa_circ_0081534 可在体外诱导 NPC 细胞增殖和侵袭减少，并在体内抑制肿瘤生长。在机制上，hsa_circ_0081534 通过海绵吸附 miR-508-5p 促进 NPC 进展。纤连蛋白 1（FN1）是 miR-508-5p 的靶基因。此外，挽救实验表明，FN1 过表达（或 miR-508-5p 抑制剂）可消除 hsa_circ_0081534 抑制对 NPC 细胞增殖和侵袭的作用。因此，hsa_circ_0081534 通过调节 miR-508-5p/FN1 轴促进 NPC 细胞的增殖和侵袭。我们的研究结果表明，hsa_circ_0081534 可能成为治疗 NPC 患者的新的治疗靶点。
