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单个甘氨酸标签可实现无金属蛋白纯化。

A single amino acid Gly-tag enables metal-free protein purification.

作者信息

Purushottam Landa, V B Unnikrishnan, Rawale Dattatraya Gautam, Gujrati Mansi, Mishra Surya Dev, T K Sajeev, Reddy Neelesh C, Adusumalli Srinivasa Rao, Mishra Ram Kumar, Rai Vishal

机构信息

Department of Chemistry, Indian Institute of Science Education and Research Bhopal Bhopal Bypass Road, Bhauri Bhopal 462 066 India

Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal Bhopal Bypass Road, Bhauri Bhopal 462 066 India.

出版信息

Chem Sci. 2020 Oct 26;11(48):13137-13142. doi: 10.1039/d0sc01153c.

DOI:10.1039/d0sc01153c
PMID:34094495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8163197/
Abstract

Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C-C bond dissociation by resonance-assisted electron density polarization.

摘要

分析纯蛋白质对于包括治疗学在内的多种应用而言不可或缺。在此，我们报告了一种方法，即单一氨基酸甘氨酸可实现无金属蛋白质纯化。这种强大的平台由一种甘氨酸标签树脂实现，该树脂通过共振辅助电子密度极化引发的化学触发碳 - 碳键解离，用于位点特异性捕获、富集和释放。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/bc76c427c5f3/d0sc01153c-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/b57399f4d76f/d0sc01153c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/6f5b4a0c5bb8/d0sc01153c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/e5284dad61eb/d0sc01153c-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/c423fc375911/d0sc01153c-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/b52adfdf834f/d0sc01153c-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/bc76c427c5f3/d0sc01153c-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/b57399f4d76f/d0sc01153c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/6f5b4a0c5bb8/d0sc01153c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/e5284dad61eb/d0sc01153c-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/c423fc375911/d0sc01153c-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/b52adfdf834f/d0sc01153c-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f16/8163197/bc76c427c5f3/d0sc01153c-f6.jpg

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引用本文的文献

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本文引用的文献

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Chemical methods for modification of proteins.蛋白质的化学修饰方法。
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2
Condensation of 2-((Alkylthio)(aryl)methylene)malononitrile with 1,2-Aminothiol as a Novel Bioorthogonal Reaction for Site-Specific Protein Modification and Peptide Cyclization.2-((烷基硫代)(芳基亚甲基)丙二腈与 1,2-氨硫醇的缩合反应作为一种新型的生物正交反应用于蛋白质定点修饰和肽环化。
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蛋白质的单一位点甘氨酸特异性标记。
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