人中性粒细胞碱性磷酸酶同工酶在琼脂糖凝胶中的等电聚焦
Isoelectric focusing of human neutrophil alkaline phosphatase isoenzymes in agarose gel.
作者信息
Brisson-Lougarre A, Vergnes H, Grozdea J, Bierme R
机构信息
Service Univesitaire d'Hématologie, Faculté de Médecine de Toulouse Rangueil, France.
出版信息
Clin Chim Acta. 1988 Jul 15;175(2):121-7. doi: 10.1016/0009-8981(88)90001-0.
An isoelectric focusing technique for human neutrophil alkaline phosphatase isoenzymes is described. After sonication with Zwittergent 3-12, butanol extraction and ultracentrifugation, dialysis of cytosols precedes focusing. Focusing patterns show a heterogeneity with two enzymatic activity areas: a main component at pI 6.7-6.8 with a minor component at pI 4.8-5.0 which is difficult to visualize due to its sensitivity to experimental conditions. The addition of 3 mmol/l ZnCl2 to the agarose gel improved the staining of focused bands and in particular the anodic component.
描述了一种用于人中性粒细胞碱性磷酸酶同工酶的等电聚焦技术。在用两性离子去污剂3-12进行超声处理、丁醇提取和超速离心后，在聚焦之前对胞质溶胶进行透析。聚焦模式显示出异质性，有两个酶活性区域：主要成分在pI 6.7-6.8，次要成分在pI 4.8-5.0，由于其对实验条件敏感，难以观察到。向琼脂糖凝胶中添加3 mmol/l氯化锌可改善聚焦条带的染色，尤其是阳极成分的染色。
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