Cleeve H J, Tua D C
Clin Chim Acta. 1984 Mar 13;137(3):333-40. doi: 10.1016/0009-8981(84)90121-9.
Human tissue alkaline phosphatases obtained by butanol treatment of tissue homogenates were subjected to isoelectric focusing in agarose gel. Inactivation of alkaline phosphatases by ampholytes was prevented by incorporating zinc into the gel prior to electrofocusing and to the authors' knowledge this has not been reported before. The addition of zinc did not alter the pH gradient and the method was reproducible. Ampholytes from two different manufacturers differed in their requirements for zinc in preventing inhibition of alkaline phosphatase. The method is more rapid than previously described methods and is very sensitive.
通过用丁醇处理组织匀浆获得的人组织碱性磷酸酶在琼脂糖凝胶中进行等电聚焦。在电聚焦之前通过向凝胶中加入锌来防止两性电解质使碱性磷酸酶失活，据作者所知，此前尚未有过此类报道。锌的添加并未改变pH梯度，且该方法具有可重复性。来自两个不同制造商的两性电解质在防止碱性磷酸酶抑制方面对锌的需求有所不同。该方法比先前描述的方法更快且非常灵敏。
