一种更简便、快速且灵敏的荧光法测定血清中的异烟肼和乙酰异烟肼。用于乙酰化酶表型分析。

A more simple, rapid and sensitive fluorimetric method for the determination of isoniazid and acetylisoniazid in serum. Application for acetylator phenotyping.

作者信息

Ioannou P C

机构信息

Chemistry Department, University of Athens, Greece.

出版信息

Clin Chim Acta. 1988 Jul 15;175(2):175-81. doi: 10.1016/0009-8981(88)90007-1.

DOI:10.1016/0009-8981(88)90007-1

PMID:3409532

Abstract

A simple, rapid and sensitive fluorimetric method for isoniazid determination in serum is described. The method is based on the reaction of isoniazid with 2-hydroxy-1-naphthaldehyde in acidic medium in the presence of excess of scandium. Within-run precision (CV) was 1.5%, 1.0% and 1.2% at mean isoniazid concentration in serum of 0.244, 1.94, and 25.9 mg/l respectively (n = 10); between-run precision (CV) was 3.0%, 2.6%, and 1.9% at mean isoniazid concentration of 0.265, 1.93, and 26.2 mg/l respectively (n = 10). The linearity of the method extended over the range of 0-300 mg isoniazid/l serum. The detection limit (defined as three times the SD of the mean blank) for the method is 0.008 mg/l of serum. Samples from 80 tuberculous patients treated with isoniazid were analysed by the proposed method (y) and by the modified spectrofluorimetric method of Miceli et al (x). Linear regression analysis of the results yielded the equation y = 0.98x + 0.05 (r = 0.986, Sxy = 0.22).

摘要

本文描述了一种用于测定血清中异烟肼的简单、快速且灵敏的荧光分析法。该方法基于在过量钪存在下，异烟肼与2-羟基-1-萘甲醛在酸性介质中的反应。当血清中异烟肼平均浓度分别为0.244、1.94和25.9 mg/l时（n = 10），批内精密度（CV）分别为1.5%、1.0%和1.2%；当异烟肼平均浓度分别为0.265、1.93和26.2 mg/l时（n = 10），批间精密度（CV）分别为3.0%、2.6%和1.9%。该方法的线性范围为0 - 300 mg异烟肼/升血清。该方法的检测限（定义为平均空白值标准差的三倍）为0.008 mg/升血清。采用所提出的方法（y）和Miceli等人改进的荧光分光光度法（x）对80例接受异烟肼治疗的结核病患者的样本进行了分析。对结果进行线性回归分析得到方程y = 0.98x + 0.05（r = 0.986，Sxy = 0.22）。