通过 C-NMR 稳定同位素分析评估线粒体调节。

Mitochondrial Regulation Assessment by C-NMR Isotopomer Analysis.

机构信息

Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal.

REQUIMTE/LAQV, Group of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal., University of Coimbra, Coimbra, Portugal.

出版信息

Methods Mol Biol. 2021;2310:259-270. doi: 10.1007/978-1-0716-1433-4_14.

DOI:10.1007/978-1-0716-1433-4_14

PMID:34096007

Abstract

Mitochondria play a central role in metabolic reprograming that occurs in numerous disease conditions. A precise evaluation of the extent of mitochondrial involvement in the metabolic alterations is essential for a better definition of metabolically based therapeutic strategies. In this chapter, some simple protocols are presented, using carbon 13 tracers and nuclear magnetic resonance isotopomer analysis, for the evaluation of mitochondrial contributions to intermediary metabolism and the metabolic effects of the implementation of some mitochondrial regulatory mechanisms.

摘要

线粒体在许多疾病状态中发生的代谢重编程中发挥着核心作用。精确评估线粒体在代谢改变中的参与程度对于更好地定义基于代谢的治疗策略至关重要。本章介绍了一些使用碳 13 示踪剂和核磁共振同位素质谱分析的简单方案，用于评估线粒体对中间代谢的贡献以及实施某些线粒体调节机制的代谢影响。