β细胞中果糖的代谢：一项基于¹³C核磁共振波谱的稳定同位素示踪研究。

Metabolism of fructose in B-cells: A C NMR spectroscopy based stable isotope tracer study.

作者信息

Ijare Omkar B, Baskin David S, Sharpe Martyn A, Pichumani Kumar

机构信息

Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX, 77030, USA.

出版信息

Anal Biochem. 2018 Jul 1;552:110-117. doi: 10.1016/j.ab.2018.04.003. Epub 2018 Apr 11.

DOI:10.1016/j.ab.2018.04.003

PMID:29654744

Abstract

Earlier studies on glucose metabolism in B-cells suggested an active TCA cycle in both naïve B cells and differentiated IgA plasma cells. Glycolysis was shown to be more active in IgA plasma cells than naïve B-cells. There have been no reports on the metabolism of fructose in B-cells. Fructose is a major sugar present in the western diet. Thus, we have investigated the metabolism of fructose in B-cells including the effect of glucose on the metabolism of fructose. In this study, using C NMR spectroscopy and [U-C]fructose and [U-C]glucose as stable C isotope tracers, we investigated the metabolic fate of fructose and glucose in B-cells. B-cells showed mitochondrial oxidation of fructose when administered alone, but showed diminished oxidation of fructose in the presence of glucose. On the other hand, fructose did not significantly affect the mitochondrial metabolism of glucose.

摘要

早期关于B细胞葡萄糖代谢的研究表明，在未成熟B细胞和分化的IgA浆细胞中，三羧酸循环均活跃。研究显示，IgA浆细胞中的糖酵解比未成熟B细胞更活跃。目前尚无关于B细胞中果糖代谢的报道。果糖是西方饮食中的主要糖类。因此，我们研究了B细胞中果糖的代谢，包括葡萄糖对果糖代谢的影响。在本研究中，我们使用碳核磁共振波谱以及[U-¹³C]果糖和[U-¹³C]葡萄糖作为稳定的碳同位素示踪剂，研究了B细胞中果糖和葡萄糖的代谢去向。单独给予果糖时，B细胞显示出线粒体对果糖的氧化作用，但在有葡萄糖存在的情况下，果糖的氧化作用减弱。另一方面，果糖对葡萄糖的线粒体代谢没有显著影响。