Amutkan Mutlu Damla, Polat Irmak, Suludere Zekiye
Faculty of Science, Department of Biology, Gazi University, Ankara06500, Turkey.
Faculty of Science, Department of Biology, Çankırı Karatekin University, Çankırı18100, Turkey.
Microsc Microanal. 2021 Jun 7:1-8. doi: 10.1017/S1431927621000404.
This study presents the oocyte development of Poecilimon ataturki Ünal, 1999 (Orthoptera, Tettigoniidae) with histology, morphology, and histochemistry by using a stereomicroscope, a light microscope, a scanning electron microscope, and a transmission electron microscope. The ovary in this species is a panoistic type which contains many ovarioles which consist of terminal filament, germarium, and vitellarium. Germarium is the region that has undifferentiated cells which generate the oocytes and follicular cells. In the vitellarium region, yolk granules start to cover the whole oocyte. In histochemical studies, to determine the content of the yolk granules, proteins, and carbohydrates in oocytes were treated with a bromophenol blue (BPB) method, a mercury bromophenol blue (mBPB) method, and a periodic acid Schiff (PAS) method, respectively. As a result of these methods, the yolk granules gave positive results in ovariole sections treated with the PAS and the BPB, while the mBPB staining was negative.
本研究运用体视显微镜、光学显微镜、扫描电子显微镜和透射电子显微镜，通过组织学、形态学和组织化学方法呈现了1999年发现的阿塔图尔克波氏螽斯（直翅目，螽斯科）的卵母细胞发育情况。该物种的卵巢为无滋式类型，包含许多卵巢小管，这些卵巢小管由端丝、生殖区和卵黄区组成。生殖区是具有未分化细胞的区域，这些细胞会产生卵母细胞和滤泡细胞。在卵黄区，卵黄颗粒开始覆盖整个卵母细胞。在组织化学研究中，为了确定卵母细胞中卵黄颗粒、蛋白质和碳水化合物的含量，分别采用了溴酚蓝（BPB）法、汞溴酚蓝（mBPB）法和过碘酸希夫（PAS）法。通过这些方法，在经PAS和BPB处理的卵巢小管切片中，卵黄颗粒呈阳性结果，而mBPB染色呈阴性。
