Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2021;2326:19-32. doi: 10.1007/978-1-0716-1514-0_2.
The contamination of heavy metals, a class of naturally occurring and persistent toxicants, has become a major public health concern due to increasing industrial and anthropogenic activities. The use of COPAS Biosort, a flow cytometer capable of measuring thousands of nematodes in minutes via high-throughput assays, has been widely applied in C. elegans studies for assessing toxicity of individual metals; however, such application yet to be seen for metals or other chemical mixtures. In the present protocol, we investigated toxic effects of individual metals, Cd, Pb, and Mn, as well as their binary and ternary mixtures, using nematode C. elegans. The toxic outcomes, including effects on growth, reproduction, and feeding behavior, were measured using high-throughput platform analysis (COAPS Biosort).
重金属污染，一类自然存在且持久的有毒物质，由于工业和人为活动的增加，已成为主要的公共卫生关注点。COPA Biosort 是一种流式细胞仪，能够通过高通量检测在几分钟内测量数千个线虫，已广泛应用于秀丽隐杆线虫研究中，以评估单个金属的毒性；然而，这种应用尚未在金属或其他化学混合物中看到。在本方案中，我们使用秀丽隐杆线虫研究了单个金属（Cd、Pb 和 Mn）及其二元和三元混合物的毒性作用。使用高通量平台分析（COAPS Biosort）测量了生长、繁殖和摄食行为等毒性结果。
