Boyd Windy A, Smith Marjolein V, Freedman Jonathan H
Biomolecular Screening Branch, National Toxicology Program, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
Methods Mol Biol. 2012;889:15-24. doi: 10.1007/978-1-61779-867-2_3.
A number of practical advantages have made the nematode Caenorhabditis elegans a useful model for genetic and developmental biological research. These same advantages, along with conservation of disease and stress response pathways, availability of mutant and transgenic strains, and wealth of biological information, have led to the increased use of C. elegans in toxicological studies. Although the potential to study the mechanisms of developmental toxicology in C. elegans is promising, embryonic and larval growth tests to identify compounds that affect the nematode have remained the primary use of C. elegans in developmental toxicology. Here, we describe a C. elegans larval growth and development assay for medium- and high-throughput screening using the COPAS Biosort flow cytometer and provide descriptions of the data and subsequent analysis.
线虫秀丽隐杆线虫具有诸多实际优势，使其成为遗传和发育生物学研究的有用模型。这些相同的优势，加上疾病和应激反应途径的保守性、突变体和转基因品系的可用性以及丰富的生物学信息，导致秀丽隐杆线虫在毒理学研究中的使用增加。尽管在秀丽隐杆线虫中研究发育毒理学机制的潜力很大，但用于鉴定影响线虫的化合物的胚胎和幼虫生长试验仍然是秀丽隐杆线虫在发育毒理学中的主要用途。在此，我们描述了一种使用COPAS Biosort流式细胞仪进行中高通量筛选的秀丽隐杆线虫幼虫生长和发育测定方法，并提供了数据描述及后续分析。
