比较 AdeABC 外排泵介导的抗药性在鲍曼不动杆菌参考菌株 ATCC 17978 和 ATCC 19606 中的差异。
Comparison of the Acinetobacter baumannii Reference Strains ATCC 17978 and ATCC 19606 in Antimicrobial Resistance Mediated by the AdeABC Efflux Pump.
机构信息
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.
出版信息
Antimicrob Agents Chemother. 2021 Jul 16;65(8):e0057021. doi: 10.1128/AAC.00570-21.
Abstract
The Acinetobacter baumannii RND efflux pump AdeABC is regulated by the 2-component regulator AdeRS. In this study, we compared the regulation and expression of AdeABC of the reference strains ATCC 17978 and ATCC 19606. A clearly stronger efflux activity was demonstrated for ATCC 19606. An amino acid substitution at residue 172 of was identified as a potential cause for differential expression of the pump. Therefore, we recommend caution with exclusively using single reference strains for research.
摘要
鲍曼不动杆菌的 RND 外排泵 AdeABC 受双组分调控系统 AdeRS 调控。在本研究中，我们比较了参考菌株 ATCC 17978 和 ATCC 19606 中 AdeABC 的调控和表达情况。结果显示，ATCC 19606 的外排活性明显更强。我们发现 172 位氨基酸的替换可能是导致泵表达差异的潜在原因。因此，我们建议在研究中谨慎使用单一的参考菌株。
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