Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.
Hunan Engineering Research Center of Skin Health and Disease, Changsha, China.
Pigment Cell Melanoma Res. 2021 Nov;34(6):1131-1137. doi: 10.1111/pcmr.12996. Epub 2021 Jun 28.
Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non-acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary acral melanoma cell cultures and related animal models. Here, we present four new human primary acral melanoma cell lines. To determine the mutational profile of the established primary melanoma cells for future targeted use, we performed exome sequencing. We next examined cell proliferation of the primary acral melanoma cells by colony-formation assays and CCK8 assay. We also evaluated the proliferative and metastatic potential of XYAM-4 in vivo. We report a detailed protocol for establishing cultured primary acral melanoma cells for Chinese patients and related animal models. We also summarize the features in our acral melanoma cell lines and the existing acral melanoma cell lines. This will provide an effective research tool for research on drug responses and individualized treatment for Chinese patients and comparative studies of melanomas between western and Chinese populations.
肢端黑色素瘤是中国患者中主要的黑色素瘤亚型。然而，目前大多数研究都集中在非肢端黑色素瘤上。大多数永生化的黑色素瘤细胞系和原代细胞都不是来自肢端黑色素瘤。此外，关于建立原代肢端黑色素瘤细胞培养物和相关动物模型的方法很少有报道。在这里，我们提出了四种新的人原代肢端黑色素瘤细胞系。为了确定建立的原代黑色素瘤细胞的突变特征，以便将来进行靶向治疗，我们进行了外显子组测序。接下来，我们通过集落形成试验和 CCK8 试验检测了原代肢端黑色素瘤细胞的增殖情况。我们还评估了 XYAM-4 在体内的增殖和转移潜能。我们报告了一种详细的建立中国患者原代肢端黑色素瘤细胞培养物和相关动物模型的方案。我们还总结了我们的肢端黑色素瘤细胞系和现有的肢端黑色素瘤细胞系的特征。这将为中国患者的药物反应和个体化治疗研究以及中西方人群黑色素瘤的比较研究提供有效的研究工具。
