Ito Takamichi, Tanaka Yuka, Tanegashima Keiko, Nishio Kiichiro, Hashimoto Hiroki, Ichiki Toshio, Ohno Fumitaka, Kaku-Ito Yumiko, Nakahara Takeshi
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Hum Cell. 2025 May 28;38(4):112. doi: 10.1007/s13577-025-01242-7.
Nail apparatus melanoma (NAM) is a specific type of cutaneous melanoma that develops in the nail apparatus of the hands and feet. The prognosis for metastatic NAM is poor due to a lack of fully effective systemic therapies. However, the difficulty in obtaining a NAM model has hindered basic research aimed at discovering effective treatment strategies. In this study, we established a NAM cell line, named KS-NailMel-1, from a primary tumor located on the nail apparatus of the left ring finger of a 68-year-old Japanese female. The cells were successfully maintained for over 9 months, exhibiting a doubling time of 38.6 ± 1.94 h. KS-NailMel-1 displayed consistent growth, spheroid formation, and invasiveness, and was confirmed to be identical to the original tumor through short tandem repeat analyses, whole-exome sequencing, and immunohistochemistry. Western blotting of the cells demonstrated the protein expression of NECTIN4, which has recently attracted attention as a potential therapeutic target for melanoma. The KS-NailMel-1 cell line represents a valuable resource for basic and preclinical research on NAM, deepening our understanding of the tumor characteristics and facilitating the development of treatment strategies for this rare form of cancer.
甲襞黑色素瘤（NAM）是一种特殊类型的皮肤黑色素瘤，发生于手足的甲襞。由于缺乏完全有效的全身治疗方法，转移性NAM的预后较差。然而，获得NAM模型的困难阻碍了旨在发现有效治疗策略的基础研究。在本研究中，我们从一名68岁日本女性左手无名指甲襞的原发性肿瘤中建立了一个NAM细胞系，命名为KS-NailMel-1。这些细胞成功维持了9个多月，倍增时间为38.6±1.94小时。KS-NailMel-1表现出持续的生长、球体形成和侵袭性，并通过短串联重复序列分析、全外显子测序和免疫组织化学证实与原始肿瘤相同。对这些细胞进行的蛋白质印迹分析显示了NECTIN4的蛋白表达，NECTIN4作为黑色素瘤的潜在治疗靶点最近受到了关注。KS-NailMel-1细胞系是NAM基础研究和临床前研究的宝贵资源，加深了我们对肿瘤特征的理解，并促进了这种罕见癌症治疗策略的开发。
