Sebring R J, Johnson N F, Spall W D
Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Cytometry. 1988 Jan;9(1):88-92. doi: 10.1002/cyto.990090114.
A new method that allows the transmission electron microscopic examination of as few as 1 x 10(4) cells obtained by flow cytometric sorting is described. The approach involves "sandwiching" fixed cells in an agarose case by a microcentrifugation system consisting of small-diameter cell-centrifugation tubes and subsequent processing of the cells by conventional techniques. The advantages offered by this method are discussed.
描述了一种新方法，该方法可对通过流式细胞术分选获得的低至1×10⁴个细胞进行透射电子显微镜检查。该方法包括通过由小直径细胞离心管组成的微量离心系统将固定细胞“夹”在琼脂糖盒中，然后用传统技术对细胞进行后续处理。讨论了该方法的优点。
