Zini N, Matteucci A, Squarzoni S, Galanzi A, Rizzoli R, Papa S
Cytometry. 1986 Nov;7(6):605-8. doi: 10.1002/cyto.990070617.
This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are discussed.
本文描述了一种有效的方法，用于浓缩通过细胞荧光分选获得的微量亚细胞颗粒，以进行电子显微镜检查。讨论了这种基于在恒定正氮压下通过密理博滤膜进行渗滤的微量方法的优点。
