McCleary Stephen, McCarthy Ronan R, Strong Rebecca, Edwards Jane, Crooke Helen
Virology Department, Animal Health and Plant Health Agency (APHA), Addlestone, KT15 3NB, United Kingdom.
J Virol Methods. 2021 Sep;295:114203. doi: 10.1016/j.jviromet.2021.114203. Epub 2021 Jun 4.
Rapid and effective virus inactivation is an essential step for safe diagnostic testing and for research and vaccine development using infectious viruses. We characterised the reduction of African Swine Fever Virus (ASFV) infectivity using Virkon™ S (Lanxess) 1% w/v disinfectant, FACS™ Lysing buffer (BD), and AVL™ buffer (Qiagen), using porcine cell culture. No virus was detected following a 30 s 20:1 v/v mixing ratio of Virkon™ S 1% with high titre ASFV, supporting its effective use as a laboratory surface disinfectant. FACS™ Lysing and AVL™ buffers also inactivated ASFV, permitting safe removal of treated infected samples from high containment facilities.
快速有效的病毒灭活是安全诊断检测以及使用传染性病毒进行研究和疫苗开发的关键步骤。我们使用猪细胞培养，对使用Virkon™ S（朗盛）1% w/v消毒剂、FACS™裂解缓冲液（BD）和AVL™缓冲液（Qiagen）降低非洲猪瘟病毒（ASFV）感染性进行了表征。1%的Virkon™ S与高滴度ASFV以20:1 v/v的混合比例混合30秒后未检测到病毒，这支持了其作为实验室表面消毒剂的有效用途。FACS™裂解缓冲液和AVL™缓冲液也能灭活ASFV，从而可以安全地从高防护设施中移除经处理的感染样本。
