Sutrisno Linawati, Chen Huajian, Chen Yazhou, Yoshitomi Toru, Kawazoe Naoki, Yang Yingnan, Chen Guoping
Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Biomaterials. 2021 Aug;275:120923. doi: 10.1016/j.biomaterials.2021.120923. Epub 2021 May 27.
Breast cancer treatment needs to eradicate cancer cells and restore breast defects after surgical intervention. Herein, bifunctional composite scaffolds of black phosphorus nanosheets (BPNSs) and gelatin were designed to kill breast cancer cells and induce adipose tissue reconstruction. The composite scaffolds were prepared by hybridizing photothermal BPNSs with porous gelatin matrices by adding pre-prepared ice particles to precisely adjust their pore structures. The composite scaffolds had large, well-interconnected spherical pores, which allowed cell migration and infiltration. Hybridization with BPNSs increased the compression strength of the scaffolds. The composite scaffolds possessed a high photothermal conversion capacity that was dependent on the amount of BPNSs. The composite scaffold with a high amount of BPNSs could completely kill breast cancer cells in vitro and in vivo under laser irradiation. Moreover, cell culture and animal experiment results showed that the composite scaffolds promoted lipid oil droplet formation and upregulated the expression of adipogenesis-related genes when hMSCs were cultured in the scaffolds. The composite scaffolds could offer a facile platform to exert anticancer effects against breast cancer cells and promote the reconstruction of adipose tissue.
乳腺癌治疗需要在手术干预后根除癌细胞并修复乳房缺损。在此，设计了黑磷纳米片（BPNSs）与明胶的双功能复合支架，以杀死乳腺癌细胞并诱导脂肪组织重建。通过将光热BPNSs与多孔明胶基质杂交，并添加预先制备的冰颗粒来精确调整其孔结构，制备了复合支架。复合支架具有大的、相互连通良好的球形孔，允许细胞迁移和浸润。与BPNSs杂交提高了支架的抗压强度。复合支架具有高光热转换能力，这取决于BPNSs的含量。含有大量BPNSs的复合支架在激光照射下可在体外和体内完全杀死乳腺癌细胞。此外，细胞培养和动物实验结果表明，当人骨髓间充质干细胞（hMSCs）在支架中培养时，复合支架促进脂质油滴形成并上调脂肪生成相关基因的表达。复合支架可以提供一个简便的平台，对乳腺癌细胞发挥抗癌作用并促进脂肪组织的重建。
