Department of Chemistry, Gebze Technical University, 41400 Gebze, Turkey.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;261:120017. doi: 10.1016/j.saa.2021.120017. Epub 2021 May 25.
A novel pyrene modified anthracene dipodal sensor was prepared by a simple synthetic method for the sensitive determination of iron ions in real samples. The chemical characterization analyses including nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were carried out to characterize the target fluorescent sensor. Photophysical and electrochemical behaviors of the sensor were studied by the absorption, excitation-emission matrix analysis, steady-state fluorescence, three-dimensional fluorescence, and cyclic and square wave voltammetry, respectively. The fluorescent sensor properties were evaluated via Ultraviolet-visible and fluorescence spectroscopies. According to obtained results, the fluorescence signal of the sensor was selectively quenched with interaction with Fe ions. The spectrofluorimetric determination of iron, in real water and medicine samples were successfully carried out under optimized experimental conditions. A detection limit and linear working range were calculated as 0.265 μM and 0.275-55.000 μM, respectively which demonstrated the ability of the simple and sensitive sensor for slight amounts of iron. The obtained detection limit for iron determination with the presented novel fluorescent sensor was less than nearly 20 times the tolerance limit (5.40 µM) in drinking water that was determined by the United States Environmental Protection Agency. The accuracy of the newly developed method was evaluated by Inductively coupled plasma optical emission spectroscopy and spike/recovery test which demonstrated that the developed fluorescent sensor has high accuracy for fast, easy and accessible determination of iron at 95% confidence level.
一种新型的芘基修饰的蒽二足传感器通过简单的合成方法制备，用于灵敏测定实际样品中的铁离子。采用核磁共振波谱、傅里叶变换红外光谱和基质辅助激光解吸/电离飞行时间质谱等化学特征分析对目标荧光传感器进行了表征。通过吸收、激发-发射矩阵分析、稳态荧光、三维荧光和循环伏安法和方波伏安法分别研究了传感器的光物理和电化学行为。通过紫外-可见和荧光光谱评估了荧光传感器的性能。根据实验结果，传感器的荧光信号通过与 Fe 离子的相互作用被选择性猝灭。在优化的实验条件下，成功地进行了实际水样和药物样品中的铁的分光光度法测定。在 0.275-55.000 μM 的线性工作范围内，计算出了检测限和线性工作范围分别为 0.265 μM 和 0.275-55.000 μM，这表明该简单灵敏的传感器能够检测痕量的铁。与美国环保署（United States Environmental Protection Agency）确定的饮用水中允许的铁含量（5.40 μM）相比，用所提出的新型荧光传感器测定铁的检测限低 20 倍左右。用电感耦合等离子体发射光谱法和加标/回收试验对新方法的准确性进行了评估，结果表明，该荧光传感器具有高准确度，可在 95%置信水平下快速、简便、可用于铁的测定。
